Effects of mutations in the adenoviral E1B 55-kilodalton protein coding sequence on viral late mRNA metabolism

Journal of Virology
Ramon Gonzalez, S J Flint

Abstract

The human subgroup C adenoviral E1B 55-kDa protein cooperates with the viral E4 Orf6 protein to induce selective export of viral, late mRNAs from the nucleus to the cytoplasm. Previous studies have suggested that such preferential transport of viral mRNA and the concomitant inhibition of export of cellular mRNAs are the result of viral colonization of specialized microenvironments within the nucleus. However, neither the molecular basis of this phenomenon nor the mechanism by which the E1B 55-kDa protein acts has been elucidated. We therefore examined viral late mRNA metabolism in HeLa cells infected with a series of mutant viruses that carry insertions at various positions in the E1B protein coding sequence (P. R. Yew, C. C. Kao, and A. J. Berk, Virology 179:795-805, 1990). All the mutations examined impaired cytoplasmic accumulation of viral L2 mRNAs and reduced L2 mRNA export efficiency. However, in most cases these defects could be ascribed to reduced E1B 55-kDa protein concentration or the unexpected failure of the altered E1B proteins to enter the nucleus efficiently. The latter property, the pleiotropic defects associated with all the mutations that impaired nuclear entry of the E1B protein, and consideration of its prim...Continue Reading

References

Nov 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·G K McMaster, G G Carmichael
Jun 1, 1992·Molecular and Cellular Biology·Y KasaiS J Flint
Dec 1, 1991·Trends in Biochemical Sciences·C Dingwall, R A Laskey
Sep 1, 1990·Journal of Virology·J K SmileyS J Flint
Jan 1, 1987·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·G Blobel
Jan 1, 1986·Advances in Virus Research·S J Flint
Apr 1, 1984·Experimental Cell Research·F Puvion-DutilleulA Macieira-Coelho
Apr 1, 1983·Molecular and Cellular Biology·C L Castiglia, S J Flint
Apr 1, 1994·The Journal of General Virology·D TakayesuP E Branton
Apr 1, 1993·The Journal of General Virology·K N Leppard
May 1, 1996·Genes & Development·L J Ko, C Prives
Oct 4, 1996·Cell·P A Baeuerle, D Baltimore
Oct 15, 1996·Proceedings of the National Academy of Sciences of the United States of America·M MooreT Shenk
Oct 1, 1996·The Journal of Cell Biology·E BridgeU Pettersson
Dec 15, 1996·Experimental Cell Research·E Bridge, U Pettersson

❮ Previous
Next ❯

Citations

Nov 1, 1994·Trends in Neurosciences·R J Rylett, L R Williams
Nov 22, 2005·Oncogene·Matthew D Weitzman, David A Ornelles
Jul 11, 2008·Journal of Virology·Rachel A SchwartzMatthew D Weitzman
Nov 3, 2006·Journal of Virology·Jennifer L Woo, Arnold J Berk
Dec 3, 2010·Journal of Virology·Gayatri YatherajamS J Flint
Feb 13, 2009·Journal of Virology·Andrew N Blackford, Roger J A Grand
Dec 16, 2011·Journal of Virology·Melanie SchmidThomas Dobner
Apr 14, 2004·Journal of Virology·Rozanne M Sandri-Goldin
Aug 17, 2005·Journal of Virology·Felipe D AraujoMatthew D Weitzman
Nov 30, 2006·Molecular and Cellular Biology·Brenden RickardsGary LeRoy
Apr 13, 2007·Proceedings of the National Academy of Sciences of the United States of America·Kathrin KindsmüllerThomas Dobner
Feb 8, 2013·The Journal of General Virology·Yadvinder S AhiSuresh K Mittal
Nov 8, 2016·Scientific Reports·Yasel GarcésJuan Manuel Rendón-Mancha
Sep 24, 2013·Molecular & Cellular Proteomics : MCP·Caroline J DeHartDavid H Perlman
Nov 27, 2019·FEBS Letters·Paloma HidalgoRamón A Gonzalez
Feb 1, 2003·The Journal of General Virology·Katherine J LethbridgeKeith N Leppard
Oct 28, 2008·Journal of Bacteriology·Michael C KonopkaJames C Weisshaar
Aug 19, 2015·Oncotarget·Christopher LarsonTony R Reid
Jul 1, 2020·Virus Research·Anna Bojagora, Vivian Saridakis

❮ Previous
Next ❯

Related Concepts

Related Feeds

Auditory Perception

Auditory perception is the ability to receive and interpret information attained by the ears. Here is the latest research on factors and underlying mechanisms that influence auditory perception.