Effects of mutations in the human immunodeficiency virus type 1 Gag gene on RNA packaging and recombination.

Journal of Virology
Olga NikolaitchikWei-Shau Hu

Abstract

Human immunodeficiency virus type 1 (HIV-1) recombination occurs during reverse transcription when parts of the two co-packaged RNAs are used as templates for DNA synthesis. It was previously hypothesized that HIV-1 Gag polyproteins preferentially encapsidate the RNA from which they were translated (cis-packaging hypothesis). This hypothesis implies that mutants encoding Gag that cannot efficiently package viral RNA are selected against at two levels: these mutants do not generate infectious virus, and these mutants are not efficiently rescued by the wild-type virus because the mutant RNAs are packaged at much lower levels than are those of the wild-type genome. Therefore, genetic information encoded by gag mutants can be rapidly lost in the viral population. To test this prediction of the cis-packaging hypothesis, we examined several gag mutants by measuring the efficiencies of the mutant RNAs in being packaged in trans in the presence of wild-type virus and determining the rates of recombination between gag mutants and wild-type viruses. We observed that the viral RNAs from the nucleocapsid zinc finger or the capsid truncation mutant were packaged efficiently in trans, and these mutant viruses also frequently recombined with ...Continue Reading

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Citations

Mar 12, 2011·Nucleic Acids Research·Ben BerkhoutTruus E M Abbink
Dec 21, 2007·Journal of Virology·Liza S Z LarsenSuzanne Sandmeyer
Sep 2, 2009·Microbiology and Molecular Biology Reviews : MMBR·Adewunmi Onafuwa-Nuga, Alice Telesnitsky
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Dec 30, 2015·Proceedings of the National Academy of Sciences of the United States of America·Jianbo ChenWei-Shau Hu
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Nov 19, 2014·Proceedings of the National Academy of Sciences of the United States of America·Jianbo ChenWei-Shau Hu
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Mar 7, 2020·Proceedings of the National Academy of Sciences of the United States of America·Jianbo ChenWei-Shau Hu
Oct 4, 2020·Viruses·Patricia S BoydSaif Yasin

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