Effects of N- and L-type calcium channel antagonists and (+/-)-Bay K8644 on nerve-induced catecholamine secretion from bovine perfused adrenal glands

British Journal of Pharmacology
M O'FarrellP D Marley

Abstract

1. The effects of N- and L-type calcium channel antagonists and (+/-)-Bay K8644 on catecholamine release from chromaffin cells and acetylcholine release from splanchnic nerve terminals was investigated in bovine perfused adrenal glands. 2. Adrenal glands were perfused retrogradely and preloaded with [3H]-choline. Subsequent efflux of 3H-labelled compounds was taken as an index of acetylcholine release from the splanchnic nerve terminals. Noradrenaline and adrenaline release from the glands was measured by h.p.l.c. with electrochemical detection. 3. A maximally effective frequency of field stimulation of the adrenal nerves, 10 Hz, induced release of catecholamines and 3H-labelled compounds. Tetrodotoxin (1 microM) abolished release of both catecholamines and 3H-labelled compounds. A combination of mecamylamine (5 microM) and atropine (1 microM) inhibited nerve-induced catecholamine release by about 75% but did not inhibit release of 3H-labelled compounds. Reducing the concentration of extracellular calcium 5 fold to 0.5 mM inhibited nerve-induced catecholamine release by 80% and release of 3H-labelled compounds by 50%. 4. (+/-)-Bay K8644 (1 microM), nitrendipine (1 microM), omega-conotoxin-GVIA (10 nM) and the combination of nit...Continue Reading

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Citations

Feb 10, 2004·Autonomic Neuroscience : Basic & Clinical·Dong-Yoon LimSoichi Miwa
Jun 24, 2004·Neurochemistry International·Tsuyoshi AkiyamaKenji Sunagawa
Feb 14, 2002·Progress in Biophysics and Molecular Biology·T E Fisher, C W Bourque
Aug 24, 1999·The European Journal of Neuroscience·E A Lukyanetz, E Neher
Dec 21, 2005·Acta Pharmacologica Sinica·Dong-yoon Lim, Il-sik Kim
Oct 16, 1999·The Journal of Physiology·T NagayamaS Satoh
Sep 9, 2008·Neuroscience Letters·Shirley A DouglasStephen J Bunn
Apr 25, 2007·Biochemical and Biophysical Research Communications·Juliana Martins RosaAntonio G García
Mar 20, 2004·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Tsuyoshi AkiyamaKenji Sunagawa
Oct 4, 2006·Physiological Reviews·Antonio G GarcíaJavier García-Sancho
Jul 13, 2001·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Y FukushimaS Satoh

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