Effects of N-acetyl-L-cysteine on lifespan, locomotor activity and stress-resistance of 3 Drosophila species with different lifespans

Aging
Mikhail ShaposhnikovAlexey Moskalev

Abstract

N-acetyl-L-cysteine (NAC) is a derivative of the sulphur-containing amino acid L-cysteine with potential anti-aging properties. We studied 3 Drosophila species with contrast longevity differences (D. virilis is longest-lived, D. kikkawai is shortest-lived and D. melanogaster has moderate lifespan) to test the effects of NAC at 8 different concentrations (from 10 nM to 100 mM) on the lifespan, stress-resistance and locomotor activity. Except the adverse effects of highest (10 mM and 100 mM) concentrations NAC demonstrated sexually opposite and male-biased effects on Drosophila lifespan, stress-resistance and locomotor activity and not satisfied the criteria of a geroprotector in terms of the reproducibility of lifespan extending effects in different model organisms. The concentration- and sex-dependent changes in the relative expression levels of the antioxidant genes (Cat/CG6871 and Sod1/CG11793) and genes involved in hydrogen sulfide biosynthesis (Cbs/CG1753, Eip55E/CG5345 and Nfs1/CG12264) suggest the involvement of hormetic mechanisms in the geroprotective effects of NAC.

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Citations

Jun 20, 2020·Genome Génome / Conseil National De Recherches Canada·Cassandra Millet-BoureimaChiara Gamberi
Mar 7, 2019·BMC Systems Biology·Alexey MoskalevMikhail Shaposhnikov
May 1, 2019·Antioxidants·Špela ŠalamonIrina Milisav
Feb 16, 2021·Journal of Food Biochemistry·Yang YueJianchu Chen
Feb 1, 2021·Ageing Research Reviews·Alexander S SokolovAlexey A Moskalev
Jan 7, 2021·Nutrition and Metabolic Insights·Florence GizardFilipe De Vadder
Jul 24, 2021·GeroScience·Mitchell B LeeMatt Kaeberlein

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Methods Mentioned

BETA
PCR
RNA Assay

Software Mentioned

QuantPrime
OASIS
CFX Manager
STATISTICA
R

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