Effects of N-acetylcystein on bleomycin-induced apoptosis in malignant testicular germ cell tumors.

Journal of Physiology and Biochemistry
Aysegul CortTomris Ozben

Abstract

Oxidative stress has been shown to induce apoptosis in cancer cells. Therefore, one might suspect that antioxidants may inhibit reactive oxygen species (ROS) and prevent apoptosis of cancer cells. No study has been carried out so far to elucidate the effects of N-acetylcysteine (NAC) on bleomycin-induced apoptosis in human testicular cancer (NCCIT) cells. We investigated the molecular mechanisms of apoptosis induced by bleomycin and the effect of NAC in NCCIT cells. We compared the effects of bleomycin on apoptosis with H(2)O(2) which directly produces ROS. Strong antioxidant NAC was evaluated alone and in combination with bleomycin or H(2)O(2) in germ cell tumor-derived NCCIT cell line (embryonal carcinoma, being the nonseminomatous stem cell component). We determined the cytotoxic effect of bleomycin and H(2)O(2) on NCCIT cells and measured apoptosis markers such as caspase-3, caspase-8, and caspase-9 activities and Bcl-2, Bax, and cytochrome c (Cyt-c) levels in NCCIT cells incubated with bleomycin, H(2)O(2), and/or NAC. We found half of the lethal dose (LD(50)) of bleomycin on NCCIT cell viability as 120 μg/ml after incubation for 72 h. Incubation with bleomycin (LD(50)) induced increases in caspase-3, caspase-8, and caspase...Continue Reading

References

Jan 1, 1993·Journal of Cellular Biochemistry. Supplement·N De Vries, S De Flora
Jan 4, 1993·Journal of Immunological Methods·D SladowskiM Balls
Jan 26, 2002·The International Journal of Biochemistry & Cell Biology·Kamal DattaParthaprasad Chattopadhyay
Jul 18, 2002·Nature Reviews. Drug Discovery·Jason Y Zhang
Jul 18, 2002·Nature Reviews. Drug Discovery·John C Reed
Sep 5, 2002·Nature Reviews. Cancer·Suzanne Cory, Jerry M Adams
Mar 24, 2004·Toxicon : Official Journal of the International Society on Toxinology·B JiangL J An
Oct 12, 2004·European Journal of Clinical Investigation·I AkanT Ozben
Oct 30, 2004·The Journal of Nutrition·Kenneth A Conklin
Jan 5, 2005·Free Radical Biology & Medicine·Thomas ZulianiMarie-Hélène Ratinaud
Apr 20, 2005·The Journal of Surgical Research·S Halide AkbasTomris Ozben
Jun 27, 2007·Journal of Pharmaceutical Sciences·Tomris Ozben
Nov 1, 2006·Expert Review of Clinical Immunology·Halide S AkbasTomris Ozben
Dec 1, 2010·Nature Reviews. Endocrinology·Christian Winter, Peter Albers
Mar 25, 2011·Molecular and Cellular Biochemistry·Ignacio BejaranoJosé A Pariente

❮ Previous
Next ❯

Citations

Apr 19, 2013·Journal of Molecular Histology·Debao LiZhiming Cui
Nov 28, 2012·Journal of Molecular Neuroscience : MN·Jinlong ZhangZhiming Cui
Aug 28, 2014·Journal of Physiology and Biochemistry·Guo Hua SongFu Bing Huang
May 8, 2014·Journal of Molecular Histology·Ya WuXiaozu Xu
Nov 20, 2014·Cellular and Molecular Neurobiology·Ming DaiAimin Sang
May 19, 2018·Journal of Cellular Biochemistry·Mahesh M Gouda, Yashodhar P Bhandary
Feb 7, 2013·Journal of Cellular Biochemistry·Ertan KucuksayanTomris Ozben
Jul 22, 2018·Current Medicinal Chemistry·Aysegul HanikogluTomris Ozben
May 4, 2017·Antioxidants & Redox Signaling·Gabriela AguileraAbel Santamaría

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptotic Caspases

Apoptotic caspases belong to the protease enzyme family and are known to play an essential role in inflammation and programmed cell death. Here is the latest research.

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis