Effects of Na saccharin feeding and urine on barrier properties of excised rat urinary bladder

Toxicology and Applied Pharmacology
J T GatzyC Harper

Abstract

When male rats of certain strains are fed a diet with 3% or more Na saccharin, their urinary bladders develop epithelial hyperplasia and a greater incidence of tumors. Since the daily dose of saccharin is high, a link between tumor formation and the disruption of urothelial physiologic and biochemical processes has been sought. We fed male and female Sprague-Dawley rats a saccharin-free or 7.5% Na saccharin diet for 1 month. Excised bladders were mounted in flux chambers and exposed to Krebs-Ringer bicarbonate solution (KRB) or urine. Bioelectric properties and 22Na, 36Cl, and [14C]mannitol or [3H]mannitol unidirectional fluxes were measured by conventional techniques. No differences were noted between bladders from male and female animals or between Na saccharin-fed animals and animals fed the saccharin-free diet. When both surfaces of the epithelium were exposed to KRB, transepithelial dc conductance fell over 4 hr to 50% of the initial value. Conductance averaged 1.4 mS/cm2. Transepithelial potential difference (PD) was usually lumen negative and averaged 0.7 mV. Unidirectional permeability coefficients for 36Cl, 22Na, and radiomannitol were symmetric, proportional to conductance, and followed a rank order compatible with un...Continue Reading

References

Aug 27, 1976·The Journal of Membrane Biology·S A Lewis, J M Diamond
Mar 1, 1986·Cancer Letters·R Hasegawa, S M Cohen
Apr 1, 1985·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·G P Schoenig, R L Anderson
Apr 1, 1985·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·G P SchoenigF W Carlborg
Apr 1, 1985·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·R A Squire
May 1, 1982·Experimental Lung Research·J T Gatzy
Feb 1, 1983·Cancer Letters·A G Renwick, J Sims
Jan 1, 1983·The Japanese Journal of Physiology·K HottaN Ito
Aug 1, 1980·Toxicology and Applied Pharmacology·T W Sweatman, A G Renwick

❮ Previous
Next ❯

Citations

Sep 1, 1995·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·S M Cohen

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bladder Carcinoma In Situ

Bladder Carcinoma In Situ is a superficial bladder cancer that occurs on the surface layer of the bladder. Discover the latest research on this precancerous condition in this feed.