Effects of nano-anatase on ribulose-1, 5-bisphosphate carboxylase/oxygenase mRNA expression in spinach

Biological Trace Element Research
Wang XumingHong Fashui

Abstract

Being a proven photocatalyst, nano-anatase is capable of undergoing electron transfer reactions under light. In previous studies, we had proven that nano-anatase improved photosynthesis and greatly promoted spinach growth. However, the molecular mechanisms of carbon reaction promoted by nano-anatase are still not clearly understood. In the present paper, reverse transcription polymerase chain reaction and Northern blotting were carried out, and the results showed that the expression of ribulose-1, 5-bisphosphate carboxylase/oxygenase (Rubisco) small subunit (rbcS) and Rubisco large subunit (rbcL) messenger RNA (mRNA) in the nano-anatase-treated spinach were obviously promoted, respectively, while the effects of bulk TiO2 were not so significant. Accordingly, the protein expression of Rubisco from the nano-anatase-treated spinach was increased by 40% compared with control. Further analysis indicated that the activity of Rubisco in the nano-anatase-treated spinach was significantly higher than the control, by up to 2.33 times, and bulk TiO2 treatment had no such significant effects. Together, one of the molecular mechanisms of carbon reaction promoted by nano-anatase is that the nano-anatase treatment results in the enhancement o...Continue Reading

References

Jun 1, 1979·The Journal of Cell Biology·N H Chua, G W Schmidt
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·G M Church, W Gilbert
Oct 1, 1994·Archives of Biochemistry and Biophysics·M E Salvucci, R R Klein
Oct 1, 1996·The Plant Journal : for Cell and Molecular Biology·K Y ToS C Chen
Apr 27, 2005·Biological Trace Element Research·Lei ZhengChao Liu
Jun 3, 2005·Biological Trace Element Research·Fashui HongPing Yang
Jul 22, 2005·Biological Trace Element Research·Fashui HongPing Yang
Feb 14, 2006·Biochemical and Biophysical Research Communications·Chao LiuZhong-rui Li
Jul 28, 2007·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Fengqing GaoFashui Hong
Jan 15, 2008·Biological Trace Element Research·Ma LinglanHong Fashui

❮ Previous
Next ❯

Citations

Jul 5, 2012·Environmental Toxicology and Chemistry·Guanling SongHuiquan Ma
May 11, 2017·Frontiers in Plant Science·Shiheng LyuDongming Pan
Feb 9, 2017·Nanoscale Research Letters·Khwaja Salahuddin Siddiqi, Azamal Husen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.