Effects of nanosecond pulse electric fields on cellular elasticity

Micron : the International Research and Review Journal for Microscopy
Diganta DuttaMichael Stacey

Abstract

We investigated the effects of a single 60 nanosecond pulsed electric field (nsPEF) of low (15 kV/cm) and high (60 kV/cm) field strengths on cellular morphology and membrane elasticity in Jurkat cells using fluorescent microscopy and atomic force microscopy (AFM). We performed force displacement measurements on cells using AFM and calculated the Young's modulus for membrane elasticity. Differential effects were observed depending upon pulsing conditions. We found that a single nsPEF of low field strength did not induce any apparent cytoskeletal breakdown and had minor morphological changes. Interestingly, force measurements and calculation of Young's modulus showed a significant decrease in membrane elasticity. A single nsPEF of high field strength induced stark morphological changes due to disruption of the actin cytoskeleton and a marked decrease in elasticity likely caused by irreversible membrane damage. We suggest that the cellular morphology is mainly dependent on stabilization by the actin cytoskeleton, while the elasticity changes are partially dependent on the cytoskeletal integrity.

References

Oct 15, 1990·Biochemical and Biophysical Research Communications·E TekleP B Chock
Dec 29, 1972·The Journal of Membrane Biology·E Neumann, K Rosenheck
Sep 6, 2001·Bioelectromagnetics·K H SchoenbachE S Buescher
Mar 18, 2004·The Journal of Biological Chemistry·Jody A WhiteStephen J Beebe
Feb 14, 2006·Biochemical and Biophysical Research Communications·Thiruvallur R GowrishankarJames C Weaver
Sep 9, 2006·Biochemical and Biophysical Research Communications·T R Gowrishankar, James C Weaver
Oct 10, 2006·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Zlatko VasilkoskiJames C Weaver
Jun 9, 2007·Archives of Biochemistry and Biophysics·Andrei G PakhomovKarl H Schoenbach
May 20, 2009·Biochemical and Biophysical Research Communications·Andrei G PakhomovKarl H Schoenbach
Jul 22, 2009·Biochemical and Biophysical Research Communications·Thomas BerghöferPeter Nick
Nov 7, 2012·Micron : the International Research and Review Journal for Microscopy·M StaceyA Beskok
Jun 13, 2013·Biochimica Et Biophysica Acta·Louise ChopinetEtienne Dague

❮ Previous
Next ❯

Citations

Mar 19, 2016·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Csaba OrbánAlfonso Blanco-Fernandez
Jul 21, 2020·Scientific Reports·Gen UrabeSunao Katsuki
Jul 1, 2020·Skin Research and Technology : Official Journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)·Se Jik HanKyung Sook Kim
Nov 27, 2019·International Journal of Molecular Sciences·Huijuan LiYulong Sun
Nov 8, 2020·Bioelectrochemistry·Evan K MassaroMichael R von Spakovsky
Nov 27, 2020·Breast Cancer : Basic and Clinical Research·Diganta DuttaSurabhi Chandra

❮ Previous
Next ❯

Related Concepts

Related Feeds

AFM in situ DNA

AFM in situ DNA describes in situ analysis (or study) of DNA using atomic force microscopy. Discover the latest research on AFM in situ DNA here.