Effects of Nanosecond Pulsed Electric Field on Intracellular NADH Autofluorescence: A Comparison between Normal and Cancer Cells

ACS Omega
Kamlesh AwasthiNobuhiro Ohta

Abstract

Intracellular fluorescence lifetime and intensity images of the endogenous fluorophore of nicotinamide adenine dinucleotide (NADH) have been observed before and after application of nanosecond pulsed electric field (nsPEF) in normal and cancer cells, that is, in Wistar-King-Aptekman rat fetus fibroblast (WFB) cells and W31 cells, which are the malignant transformed cells from WFB. The application of nsPEF induces a change both in intensity and lifetime of NADH, indicating that the intracellular function is affected by application of nsPEF in both normal and cancer cells. The application of nsPEF induces an increase in the fluorescence lifetime of NADH and a morphological change, which is attributed to the induction of apoptosis by nsPEF. The field effect on the intensity and lifetime clearly depends on the pulse width, and magnitude of the field-induced increase in the fluorescence lifetime of NADH has a tendency to increase with a decreasing pulse width. It is also found that apoptosis can be induced only in cancer cells using a suitable nsPEF, showing a possibility that ultrashort pulsed electric field is applicable for drug-free cancer therapy.

References

Feb 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·J R LakowiczM L Johnson
Aug 28, 1998·Science·D R Green, J C Reed
Apr 20, 2002·Biophysical Journal·Shaohui HuangWatt W Webb
Feb 22, 2005·Current Opinion in Biotechnology·Horst Wallrabe, Ammasi Periasamy
Nov 29, 2007·Proceedings of the National Academy of Sciences of the United States of America·Melissa C SkalaNirmala Ramanujam
Jun 6, 2008·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Takakazu NakabayashiNobuhiro Ohta
Oct 7, 2008·Cancer Biology & Therapy·Francis G Blankenberg
Mar 10, 2009·Current Opinion in Biotechnology·James A LevittKlaus Suhling
Apr 2, 2010·Chemical Reviews·Mikhail Y Berezin, Samuel Achilefu
Mar 8, 2011·Cell·Douglas Hanahan, Robert A Weinberg
Jul 23, 2011·The Journal of Physical Chemistry. B·Shinya OgikuboNobuhiro Ohta
May 25, 2012·Journal of Microscopy·W Becker
May 24, 2014·Nature Reviews. Cancer·Saskia I J Ellenbroek, Jacco van Rheenen
Sep 16, 2014·Journal of Biophotonics·Wenbo WangHaishan Zeng
Nov 26, 2015·Accounts of Chemical Research·Kim Truc Nguyen, Yanli Zhao
Apr 20, 2016·Journal of Biophotonics·Andrew J BowerStephen A Boppart
Nov 15, 2016·Journal of Photochemistry and Photobiology. B, Biology·Kamlesh AwasthiNobuhiro Ohta

❮ Previous
Next ❯

Citations

Sep 15, 2021·The Journal of Physical Chemistry. B·Qi YangTakakazu Nakabayashi

❮ Previous
Next ❯

Methods Mentioned

BETA
fluorescence lifetime microscopy
chip

Software Mentioned

SPC image

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.