PMID: 9171091Jun 15, 1997Paper

Effects of natural and synthetic polyamines on the conformation of an oligodeoxyribonucleotide with the estrogen response element

Nucleic Acids Research
T ThomasT Thomas

Abstract

We studied the effects of natural and synthetic polyamines on the conformation of an oligodeoxyribonucleotide (ODN1) harboring the estrogen response element (ERE) by circular dichroism (CD) spectroscopy and polyacrylamide gel electrophoresis. Putrescine and spermidine had no marked effect on the CD spectrum of ODN1. In contrast, spermine provoked and stabilized two characteristic changes in the CD spectrum. The first change was indicated by an increase in the intensity of the CD band at 280 nm at 0.5 mM spermine in Tris-HCl buffer containing 50 mM NaCl. This change appears to be related to changes in base tilt and conformational alterations similar to A-DNA. At 1-2 mM spermine, the CD spectrum was characterized by a loss of positive bands at 220 and 270 nm. This change might have contributions from polyamine-induced condensation/aggregation of DNA. Spectral measurements were also conducted in Tris-HCl buffer containing 150 mM NaCl to minimize contributions from condensation and aggregation of ODN1. Under these conditions, CD spectral changes were retained by (ODN1), although the magnitude of the change was diminished. In contrast, a control oligdeoxyribonucleotide (ODN2) having similar base composition did not show any signific...Continue Reading

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Citations

Jun 29, 2007·European Biophysics Journal : EBJ·Max A Keniry, Elisabeth A Owen
Feb 16, 2005·Anti-cancer Drugs·Yi HuangNancy E Davidson
Sep 30, 2014·Journal of Chromatography. a·Ângela SousaJoão A Queiroz
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Dec 5, 2019·Organic & Biomolecular Chemistry·Mayu EsumiMakiko Tanaka
Feb 28, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Wenpan LiHaiyang Hu
Dec 25, 2016·The Journal of Chemical Physics·Akira MuramatsuKenichi Yoshikawa
Oct 24, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Oxana B KazakovaAlexander A Spasov

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