Effects of natural eggshell membrane (NEM) on cytokine production in cultures of peripheral blood mononuclear cells: increased suppression of tumor necrosis factor-α levels after in vitro digestion

Journal of Medicinal Food
Kathleen F BensonGitte S Jensen

Abstract

Tumor necrosis factor-α (TNF-α) plays an important role in inflammatory processes. This study examined the effects of natural eggshell membrane (NEM(®)) (ESM Technologies, LLC, Carthage, MO, USA) on interleukin (IL)-2, IL-4, IL-6, IL-10, interferon-γ (IFN-γ), and TNF-α cytokine production by 4-day peripheral blood mononuclear cell (PBMC) cultures exposed to serial dilutions of either an aqueous extract of natural eggshell membrane (NEM-AQ) or NEM subjected to in vitro digestion (NEM-IVD). The effects on cytokine production were also assessed in the presence of phytohemagglutinin (PHA) and pokeweed mitogen (PWM) where exposure to NEM-AQ resulted in reduced levels of proliferation and statistically significant effects on IL-6, IL-10, IFN-γ, and TNF-α cytokine production. NEM-AQ reduced levels of IL-6, IL-10, IFN-γ, and TNF-α in cultures exposed to PHA. In cultures containing PWM, NEM-AQ reduced production of IL-10 and at the highest dose tested increased IL-6 and decreased TNF-α cytokine levels. NEM-IVD, at the two lowest concentrations of product, significantly reduced TNF-α production by PBMC cultures exposed to PWM compared with the in vitro digest control or native NEM. Taken together, these results suggest that NEM-AQ can in...Continue Reading

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Citations

Dec 17, 2014·International Journal of Molecular Sciences·Yaning ShiYoshinori Mine
Jun 12, 2016·Materials Science & Engineering. C, Materials for Biological Applications·Mahesh Kumar Sah, Subha Narayan Rath
Jun 8, 2017·Food & Function·B BaspinarA O Ozcelik
Apr 27, 2021·Journal of Controlled Release : Official Journal of the Controlled Release Society·Aline Martins Dos SantosMarlus Chorilli

Methods Mentioned

BETA
electrophoresis
size-exclusion filtration
flow cytometry
size-exclusion centrifugation
size exclusion centrifugation

Related Concepts

Anti-Inflammatory Agents
Egg Shell
SDS-PAGE
Interferon Type II
RU 49637
Nongranular Leukocyte
Phytohemagglutinins
Tumor Necrosis Factor-alpha
Interleukin-4
Interleukin-6

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