Effects of Nd:YAG-laser irradiation on monolayer cell cultures

Lasers in Surgery and Medicine
N GutknechtF Lampert

Abstract

The clinical applications of Nd:YAG lasers on oral soft tissues include a wide field of surgical and periodontal procedures. This in vitro study focuses on the histological effects of Nd:YAG-laser irradiation on a fibroblast monolayer cell culture especially with regard to thermal damage and cell necrosis. The results of this basic research study provide us with clear power settings for a safe soft tissue laser treatment. Two hundred forty multiwell cell cultures and 24 micro-slide Leighton tubes were laser treated. Laser irradiation was performed with a commercial free-running pulse Nd:YAG laser and a quartz fiber with a diameter of 200 microns on L-929 fibroblast cell cultures. The variable parameters were pulse energy (30-120 mJ), pulse rate (20-100 Hz), power output (1.5-3.0 W), and time of irradiation (10-60 s). The cultures were analyzed with help of vital staining, autoradiography, and cytomorphology examination. Depending on the different settings the laser irradiation caused inhibitions of the DNA metabolism rate and the cell division rate, a degeneratively changed cytomorphology up to cell pyknosis. An increasing pulse energy, pulse rate, or an increased time of irradiation created an extended diameter of the pyknotic...Continue Reading

References

Nov 1, 1992·Journal of Dental Research·A LinR D Davidson
Jan 1, 1991·Lasers in Surgery and Medicine·J M WhiteC L Rose
Jan 1, 1989·Dermatologica·M LandthalerD Haina
Jan 1, 1995·Lasers in Surgery and Medicine·H A WigdorJ L Waldvogel
Jan 1, 1994·Lasers in Surgery and Medicine·R H OssoffL Reinisch
Jan 1, 1994·Lasers in Surgery and Medicine·J G GallucciG J Slotman

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Citations

Nov 3, 2005·Journal of Obstetrics and Gynaecology : the Journal of the Institute of Obstetrics and Gynaecology·J ErianT Theodoridis
Jun 20, 2001·Lasers in Surgery and Medicine·M KreislerB d'Hoedt
Feb 3, 2005·Lasers in Surgery and Medicine·Yi-Jane ChenWan-Hong Lan
Nov 23, 2012·International Journal of Paediatric Dentistry·Peter De CosterLuc Martens
Dec 29, 2000·Lasers in Surgery and Medicine·Y J ChenW H Lan
Jul 1, 2017·Journal of Cosmetic and Laser Therapy : Official Publication of the European Society for Laser Dermatology·Mehmet SağlamŞükrü Enhoş
Dec 1, 2007·Lasers in Medical Science·Hidetaka MiyazakiKen Omura

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