PMID: 29757584May 15, 2018Paper

Effects of Nerve Growth Factor shRNA Inhibition on Asthma Phenotypes in a Mouse Model of Asthma

Iranian Journal of Allergy, Asthma, and Immunology
Jinhong WuYanan Xiao

Abstract

Nerve growth factor (NGF) plays an important role in airway hyper-responsiveness (AHR). In this study, we aimed at investigating the effect of NGF inhibition on AHR and other asthma phenotypes in a mouse model of asthma. 12 mice in each group were injected with lentiviral vectors expressing non-targeting shRNA (sham shRNA), targeting NGF (shRNA-1 and shRNA-2), or normal saline for control before the asthma models were established. Peak inspiratory pressure (PIP), NGF levels in bronchoalveolar lavage fluid (BALF), and bronchoconstriction in response to acetylcholine (ACh) were measured. Immunohistochemistry semi-quantitative analysis of muscarinic acetylcholine receptor M3 (mAChR M3) and alpha-smooth muscle actin (a-SMA) were measured by Image Pro Plus (IPP), and qRT-PCR analysis of mRNAs of cholinergic receptors, muscarinic 3 (Chrm3), Ngf and Tropomyosin receptor kinase A (TrkA) were performed. Immunohistochemistry showed mAChR M3 was overexpressed and a-SMA was hyperplasia in control and sham shRNA, semi-quantitative analysis revealed optical density (OD) values were significantly higher than shRNA-1 and shRNA-2, (p<0.001). BALF NGF levels were significantly higher in control and sham shRNA (457.16±45.32, 676.43±111.64) compar...Continue Reading

Related Concepts

Related Feeds

Asthma

This feed focuses in Asthma in which your airways narrow and swell. This can make breathing difficult and trigger coughing, wheezing and shortness of breath.

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.

Attention Disorders

Attention is involved in all cognitive activities, and attention disorders are reported in patients with various neurological diseases. Here are the latest discoveries pertaining to attention disorders.