Effects of new beta-type Ti-40Nb implant materials, brain-derived neurotrophic factor, acetylcholine and nicotine on human mesenchymal stem cells of osteoporotic and non osteoporotic donors

PloS One
Vivien KauschkeKatrin Susanne Lips

Abstract

Treatment of osteoporotic fractures is still challenging and an urgent need exists for new materials, better adapted to osteoporotic bone by adjusted Young's modulus, appropriate surface modification and pharmaceuticals. Titanium-40-niobium alloys, mechanically ground or additionally etched and titanium-6-aluminium-4-vanadium were analyzed in combination with brain-derived neurotrophic factor, acetylcholine and nicotine to determine their effects on human mesenchymal stem cells in vitro over 21 days using lactate dehydrogenase and alkaline phosphatase assays, live cell imaging and immunofluorescence microscopy. Cell number of human mesenchymal stem cells of osteoporotic donors was increased after 14 d in presence of ground titanium-40-niobium or titanium-6-aluminium-4-vanadium, together with brain-derived neurotrophic factor. Cell number of human mesenchymal stem cells of non osteoporotic donors increased after 21 d in presence of titanium-6-aluminium-4-vanadium without pharmaceuticals. No significant increase was measured for ground or etched titanium-40-niobium after 21 d. Osteoblast differentiation of osteoporotic donors was significantly higher than in non osteoporotic donors after 21 d in presence of etched, ground titaniu...Continue Reading

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Citations

Jun 28, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Vivien KauschkeKatrin Susanne Lips
Sep 16, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Thomas Leonhard LoyKatrin Susanne Lips
Sep 16, 2020·International Journal of Molecular Sciences·Karin Wuertz-KozakPia M Wippert
May 18, 2021·Frontiers in Physiology·Laura Gerosa, Giovanni Lombardi

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Methods Mentioned

BETA
Fluorescence Activated Cell Sorting
FCS
Assay

Software Mentioned

SPSS

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