Effects of new-generation inhibitors of the calcium-activated chloride channel anoctamin 1 on slow waves in the gastrointestinal tract.

British Journal of Pharmacology
Sung Jin HwangS M Ward

Abstract

High-throughput screening of compound libraries using genetically encoded fluorescent biosensors has identified several second-generation. low MW inhibitors of the calcium-activated chloride channel anoctamin 1 (CaCC/Ano1). Here we have (i) examined the effects of these Ano1 inhibitors on gastric and intestinal pacemaker activity; (ii) compared the effects of these inhibitors with those of the more classical CaCC inhibitor, 5-nitro-2-(3-phenylpropylalanine) benzoate (NPPB); (ii) examined the mode of action of these compounds on the waveform of pacemaker activity; and (iii) compared differences in the sensitivity between gastric and intestinal pacemaker activity to the Ano1 inhibitors. Using intracellular microelectrode recordings of gastric and intestinal muscle preparations from C57BL/6 mice, the dose-dependent effects of Ano1 inhibitors were examined on spontaneous electrical slow waves. The efficacy of second-generation Ano1 inhibitors on gastric and intestinal pacemaker activity differed significantly. Antral slow waves were more sensitive to these inhibitors than intestinal slow waves. CaCCinh -A01 and benzbromarone were the most potent at inhibiting slow waves in both muscle preparations and more potent than NPPB. Dichlor...Continue Reading

References

Feb 16, 2005·Annual Review of Physiology·Criss HartzellJorge Arreola
Aug 23, 2005·Proceedings of the American Thoracic Society·Jan Eggermont
Jan 29, 2008·The American Journal of Surgical Pathology·Inigo EspinosaMatt van de Rijn
Jul 1, 2008·Developmental Biology·Jason R RockBrian D Harfe
Sep 23, 2008·Cell·Björn Christian SchroederLily Yeh Jan
Apr 18, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·Pedro J Gomez-PinillaGianrico Farrugia
Jul 17, 2009·The American Journal of Surgical Pathology·Markku MiettinenJerzy Lasota
Aug 15, 2009·The Journal of Biological Chemistry·Jiraporn OusingsawatKarl Kunzelmann
Jul 24, 2010·British Journal of Pharmacology·Carol KilkennyUNKNOWN NC3Rs Reporting Guidelines Working Group
Aug 13, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Wan NamkungA S Verkman
Oct 18, 2011·Experimental Physiology·Kenton M SandersSean M Ward
Oct 19, 2011·The Journal of Physiology·Poong-Lyul RheeSean M Ward
Aug 7, 2012·Cell and Tissue Research·Peter J BlairSean M Ward
Sep 6, 2012·British Journal of Pharmacology·Alison J DavisIain A Greenwood
Sep 19, 2012·Proceedings of the National Academy of Sciences of the United States of America·Fen HuangJason R Rock
Oct 5, 2012·American Journal of Physiology. Cell Physiology·Abigail S ForrestNormand Leblanc
Feb 23, 2013·Proceedings of the National Academy of Sciences of the United States of America·Adrian BritschgiMohamed Bentires-Alj
Nov 16, 2013·Nucleic Acids Research·Adam J PawsonUNKNOWN NC-IUPHAR
Jan 10, 2014·The Journal of Clinical Investigation·Christoph HeinzeChristian A Hübner
Feb 5, 2014·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Podchanart WanitchakoolKarl Kunzelmann
Jun 13, 2014·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Daniel J ShiwarskiUmamaheswar Duvvuri
Jul 6, 2014·Physiological Reviews·Kenton M SandersSang Don Koh
Apr 8, 2015·Proceedings of the National Academy of Sciences of the United States of America·Yasunori TakayamaMakoto Tominaga
May 13, 2015·British Journal of Pharmacology·John C McGrath, Elliot Lilley
Jun 27, 2015·British Journal of Pharmacology·Michael J CurtisJohn C McGrath
Dec 10, 2015·British Journal of Pharmacology·Stephen Ph AlexanderUNKNOWN CGTP Collaborators
Dec 10, 2015·British Journal of Pharmacology·Stephen Ph AlexanderCgtp Collaborators

❮ Previous
Next ❯

Citations

Feb 13, 2018·The Journal of Physiology·Tae Sik SungSean M Ward
Dec 10, 2019·American Journal of Physiology. Gastrointestinal and Liver Physiology·Sean P Parsons, Jan D Huizinga
May 18, 2020·The Journal of Physiology·Nathan GraingerKenton M Sanders
Mar 14, 2019·The Journal of General Physiology·Scott D ZawiejaMichael J Davis
Dec 17, 2016·American Journal of Physiology. Gastrointestinal and Liver Physiology·John MalyszGianrico Farrugia
Jul 25, 2017·Pflügers Archiv : European journal of physiology·Stephen FediganGerard P Sergeant
Jun 26, 2018·Journal of Smooth Muscle Research = Nihon Heikatsukin Gakkai Kikanshi·Shunsuke HyugaGeorge Gallos
May 28, 2019·The Journal of Physiology·Bernard T DrummKenton M Sanders
Dec 6, 2018·Journal of Cellular Physiology·Qiushuang JiHailong An
Feb 9, 2021·American Journal of Physiology. Renal Physiology·Bernard T DrummGerard P Sergeant
Jan 21, 2021·American Journal of Physiology. Cell Physiology·Minkyoung KimKyungpyo Park
Apr 1, 2021·American Journal of Physiology. Cell Physiology·Ji Yeon LeeSang Don Koh

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.