PMID: 11904622Mar 21, 2002Paper

Effects of nicotine on intercellular adhesion molecule expression in endothelial cells and integrin expression in neutrophils in vitro

American Journal of Obstetrics and Gynecology
Paul SpeerYuping Wang

Abstract

We previously reported that nicotine decreases leukocyte adhesion to uterine vascular endothelial cells in vivo under ischemic conditions in pregnant rabbits. To further investigate the mechanism of decreased leukocyte-endothelial adhesion by nicotine exposure, the effect of nicotine on endothelial cell intercellular adhesion molecule expression and neutrophil integrin expression of CD62L, CD11a, and CD11b were examined. Endothelial cells were isolated from human umbilical cord veins from normal pregnancies in nonsmoking women immediately after delivery. Neutrophils were isolated from healthy nonpregnant and nonsmoking female volunteers. First passage of endothelial cells and fresh isolated neutrophils were exposed to nicotine at different concentrations. Surface adhesion molecule expression of intercellular adhesion molecule on endothelial cells was determined by colorimetric assay. Neutrophil integrin expressions for CD62L, CD11a, and CD11b were determined by flow cytometry. Messenger RNA expression for intercellular adhesion molecule in endothelial cells was examined by reverse transcription-polymerase chain reaction. Nicotine at a lower concentration of 0.01 micromol/L had no effect on endothelial cell surface intercellular...Continue Reading

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Citations

Jul 17, 2008·The Journal of Infectious Diseases·Ilona A J GiebelenTom van der Poll
Jan 18, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Shayna T BradfordAnuska V Andjelkovic
Jul 27, 2010·American Journal of Respiratory and Critical Care Medicine·Sreekumar G PillaiUNKNOWN ECLIPSE and ICGN Investigators
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Jun 3, 2020·Vaccines·Sandra JagdmannChristian Meisel
Aug 10, 2017·Journal of Neurochemistry·Matthew J Mulcahy, Henry A Lester
May 17, 2015·American Journal of Physiology. Lung Cellular and Molecular Physiology·Kelly S SchweitzerIrina Petrache
Mar 24, 2020·International Immunopharmacology·Ignaz Wessler, Charles James Kirkpatrick
Jul 30, 2021·Biochemical Pharmacology·Abdalla M WednMahmoud M El-Mas

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