PMID: 15226598Jul 1, 2004Paper

Effects of nitric oxide and tumor necrosis factor-alpha on production of prostaglandin F2alpha and E2 in bovine endometrial cells

The Journal of Reproduction and Development
Izabela Woclawek-PotockaDariusz J Skarzynski

Abstract

The purpose of this study was to determine whether nitric oxide (NO) mediates tumor necrosis factor (TNF)alpha influence on the bovine endometrium. TNFalpha influence on the bovine endometrium is limited to the stromal cells. Therefore, it was interesting to find out whether NO production by the stromal cells, stimulated by TNFalpha might influence the endometrial epithelium. Moreover, we investigated the intracellular mechanisms of TNFalpha- and NO-regulated prostaglandin (PG) F(2alpha) and PGE(2) synthesis. Epithelial and stromal cells from the bovine endometrium (Days 2-5 of the oestrous cycle) were separated by means of enzymatic dispersion and cultured for 6-7 days in 48-well plates. The confluent endometrial cells were exposed to a NO donor (S-NAP; 1-1000 microM) for 24 h. S-NAP strongly stimulated PGE(2) production in both bovine endometrial cell types (P<0.001). The effect of SNAP on PGF(2alpha) production was limited only to the stromal cells (P<0.05). To study the intracellular mechanisms of TNFalpha and NO action, stromal cells were incubated for 24 h with TNFalpha or S-NAP and with NO synthase (NOS) inhibitor (L-NAME; 10 microM) or an inhibitor of phosphodiesterase (IBMX; 10 microM). When the cells were exposed to T...Continue Reading

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Aug 9, 2003·Biology of Reproduction·Dariusz J SkarzynskiKiyoshi Okuda
Feb 18, 2004·The Journal of Reproduction and Development·Shuko MurakamiKiyoshi Okuda

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Citations

Sep 12, 2006·The Journal of Reproduction and Development·Izabela Woclawek-PotockaDariusz J Skarzynski
Aug 2, 2008·Reproduction, Fertility, and Development·Rosário P Roberto da CostaGraça Ferreira-Dias
Oct 19, 2012·Reproduction, Fertility, and Development·A GalvãoG Ferreira-Dias
Apr 17, 2014·Animal Science Journal = Nihon Chikusan Gakkaihō·Hanako BaiKazuhiko Imakawa
Dec 3, 2014·Animal Reproduction Science·J M Carrera-ChávezC F Aréchiga-Flores
Sep 7, 2016·Domestic Animal Endocrinology·M ŁupickaA J Korzekwa
Apr 22, 2010·Experimental Biology and Medicine·Takehito SuzukiTatsuya Takizawa
Sep 30, 2015·Reproductive Biology and Endocrinology : RB&E·Martyna ŁupickaAnna Korzekwa

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