Effects of nitric oxide donors and inhibitors of nitric oxide signalling on endothelin- and serotonin-induced contractions in human placental arteries

Acta Physiologica Scandinavica
A E SandG Fried

Abstract

In order to explore the role of nitric oxide (NO) in the control of fetoplacental vascular tone in normal pregnancy we have examined the effects of NO donors on uteroplacental arteries pre-contracted with the vasoconstrictor endothelin-1 (ET-1) or serotonin (5-HT). We have furthermore examined the effects of guanylate cyclase inhibitors on the NO-induced relaxation. Segments of placental arteries (n=102) obtained from 39 placentas immediately after delivery were mounted in organ baths and superfused with Krebs-Ringer solution at 37 degrees C. The vessel segments were exposed to drugs for various intervals and the tension was recorded isometrically and registered on a polygraph. Cyclic guanosine monophosphate (cGMP) analysis was performed after extraction of vessel segments using a specific radioimmunoassay. The placental artery segments responded to ET-1 and 5-HT with a dose-dependent vasoconstriction. After pre-contraction with ET-1 (10(-7) M) or 5-HT (10(-6) M), the vessels relaxed in response to the NO donors glyceryltrinitrate (GTN) (10(-6) M) and S-nitroso-N-acetyl-penicillamine (SNAP) (10(-5) M). In the presence of the non-specific guanylate cyclase inhibitor LY 83583 (10(-6) M), the vessels responded with a small contrac...Continue Reading

References

Aug 1, 1991·European Journal of Clinical Investigation·S Moncada, E A Higgs
Jan 1, 1988·Journal of Cardiovascular Pharmacology·S A Waldman, F Murad
Jan 1, 1989·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·L J Ignarro
Dec 30, 1993·The New England Journal of Medicine·S Moncada, A Higgs
May 24, 1994·Proceedings of the National Academy of Sciences of the United States of America·C P WeinerS Moncada
Sep 1, 1995·American Journal of Obstetrics and Gynecology·K M WieczorekL Myatt
Sep 1, 1995·American Journal of Obstetrics and Gynecology·F LyallI A Greer
Aug 15, 1995·European Journal of Pharmacology·D LuoS R Vincent
Jan 26, 1993·Biochemical Pharmacology·B MayerK Schmidt
Jun 30, 1993·Biochemical and Biophysical Research Communications·T OguraH Esumi
Feb 1, 1996·American Journal of Obstetrics and Gynecology·J G Learmont, L Poston
Jan 1, 1996·Gynecologic and Obstetric Investigation·T NobunagaF Saji
Dec 15, 1995·The Journal of Physiology·S D KohK M Sanders
Apr 15, 1997·Annals of the New York Academy of Sciences·S Moncada
Apr 8, 1998·The American Journal of Physiology·R A Word, T L Cornwell
Dec 9, 1998·The American Journal of Physiology·K K BradleyM E Bradley
Sep 9, 2000·Biochemistry·Y ZhaoM A Marletta
Oct 4, 2000·British Journal of Pharmacology·F Lovren, C Triggle

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Citations

Jul 13, 2011·Naunyn-Schmiedeberg's Archives of Pharmacology·Vítor S FernandesMedardo Hernández
Dec 2, 2005·Journal of Perinatal Medicine·Kiyomi TsukimoriHitoo Nakano
Jan 23, 2016·Clinical and Experimental Hypertension : CHE·Emine IrgeEmre Karakus
Aug 12, 2008·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Brett L JenningsJohn A Donald
Sep 24, 2004·BJOG : an International Journal of Obstetrics and Gynaecology·Rosario D'AnnaFrancesco Squadrito
Dec 23, 2004·Prenatal Diagnosis·Torvid Kiserud, Ganesh Acharya
Oct 21, 2005·Seminars in Fetal & Neonatal Medicine·Torvid Kiserud
Jul 30, 2011·Placenta·B J KrauseP Casanello
Aug 18, 2006·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Mark WareingGregor K Fyfe
Apr 19, 2015·Current Opinion in Pediatrics·Ahmed SaidAllan Doctor
Oct 29, 2004·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·H W KimA G Greenburg

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