Effects of non-ablative fractional erbium glass laser treatment on gene regulation in human three-dimensional skin models

Lasers in Medical Science
Philipp M AmannJens M Baron

Abstract

Clinical experiences with non-ablative fractional erbium glass laser therapy have demonstrated promising results for dermal remodelling and for the indications of striae, surgical scars and acne scars. So far, molecular effects on human skin following treatment with these laser systems have not been elucidated. Our aim was to investigate laser-induced effects on skin morphology and to analyse molecular effects on gene regulation. Therefore, human three-dimensional (3D) organotypic skin models were irradiated with non-ablative fractional erbium glass laser systems enabling qRT-PCR, microarray and histological studies at same and different time points. A decreased mRNA expression of matrix metalloproteinases (MMPs) 3 and 9 was observed 3 days after treatment. MMP3 also remained downregulated on protein level, whereas the expression of other MMPs like MMP9 was recovered or even upregulated 5 days after irradiation. Inflammatory gene regulatory responses measured by the expression of chemokine (C-X-C motif) ligands (CXCL1, 2, 5, 6) and interleukin expression (IL8) were predominantly reduced. Epidermal differentiation markers such as loricrin, filaggrin-1 and filaggrin-2 were upregulated by both tested laser optics, indicating a pot...Continue Reading

References

May 21, 1999·The Journal of Surgical Research·K M BullardM J Banda
Feb 24, 2001·The Journal of Burn Care & Rehabilitation·R TsouN S Gibran
Dec 18, 2007·The International Journal of Biochemistry & Cell Biology·Sean E Gill, William C Parks
Jun 17, 2009·Journal of Cosmetic Dermatology·Jordan P FarkasJeffrey M Kenkel
Jan 22, 2010·Skin Pharmacology and Physiology·M M NeisH F Merk
Sep 22, 2010·The British Journal of Dermatology·J S OrringerG Fisher
Oct 15, 2010·Journal of Cellular Physiology·JianFei WangEdward E Tredget
Jan 14, 2011·Skin Research and Technology : Official Journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)·Doris Helbig, Uwe Paasch
Aug 5, 2011·Journal of the European Academy of Dermatology and Venereology : JEADV·G-Y LeeW-S Kim
Dec 20, 2011·The Journal of Allergy and Clinical Immunology·Christian CornelissenJens M Baron
Jul 5, 2012·Skin Pharmacology and Physiology·R HeiseJ M Baron
Nov 20, 2012·Indian Journal of Plastic Surgery : Official Publication of the Association of Plastic Surgeons of India·Jumaat Mohd Yussof ShahSuneet Sood
Mar 1, 2013·Plastic and Reconstructive Surgery·Paulo Afonso Monteiro Pacheco GuimarãesLydia Masako Ferreira
Jan 1, 2014·Advanced Drug Delivery Reviews·Stephanie H MathesUrsula Graf-Hausner
Jan 23, 2014·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·Kabir SardanaVivek Sagar
Mar 13, 2014·Advanced Drug Delivery Reviews·Anna Astashkina, David W Grainger
Oct 14, 2014·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·Hoda MoneibMarwa M Fawzy
Jan 15, 2015·Lasers in Surgery and Medicine·Lisa Y XuMathew M Avram

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Citations

Feb 7, 2019·Journal of Biomedical Materials Research. Part a·Franziska KreimendahlJens Malte Baron
Jun 29, 2021·Plastic and Reconstructive Surgery·Mimi R BorrelliH Peter Lorenz

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