Effects of non-ionic solute stresses on biofilm formation and lipopolysaccharide production in Escherichia coli O157:H7

Research in Microbiology
Jinki YeomWoojun Park

Abstract

The addition of non-ionic solutes such as sucrose and polyethylene glycol (PEG) to a culture of Escherichia coli O157:H7 stimulated formation of a biofilm on an abiotic surface. Possible factors involved in this increased biofilm formation were evaluated, i.e. oxidative stress, exopolysaccharide (EPS) production, membrane composition and lipopolysaccharide (LPS) production. A green fluorescent protein (GFP)-based reporter strain, anaerobic experiment and microarray data suggested that the increased biofilm formation was not due to oxidative stress. Quantification of the EPS revealed that cell-released EPS production appeared not to be related. Bacterial results of fatty acid methyl ester (FAME) analysis, along with microarray data, showed that sucrose and PEG could induce membrane rigidity via alterations in the fatty acid (FA) composition. Based on transcriptome analysis, PEG was observed to induce several membrane-related genes and membrane-associated LPS synthesis genes, confirmed by quantitative real-time RT-PCR analysis. Interestingly, biofilm cells showed higher expression than planktonic cells of ompC (encoding an outer membrane protein) and many LPS- and polysaccharide-related genes (glmS, dxs, msbB and kdsA genes) when...Continue Reading

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Citations

Dec 29, 2013·Microbial Ecology·Lizziane Kretli WinkelströterElaine Cristina Pereira De Martinis
Sep 14, 2014·Microbiology·Zoran JaglicSusanne Knøchel
Oct 26, 2016·Journal of Food Science and Technology·Jamuna Bai A, Ravishankar Rai V
Dec 20, 2016·Journal of Food Science·Nicholas RibaudoZuyi Jacky Huang
Nov 25, 2016·Environmental Microbiology·Koji YaharaSamuel K Sheppard

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