Effects of Novel Laser Dental Implant Microtopography on Human Osteoblast Proliferation and Bone Deposition

The International Journal of Oral & Maxillofacial Implants
Filiberto MastrangeloA Piattelli

Abstract

The aim of this study was to compare how two innovative laser titanium surfaces and sandblasted and acid-etched surfaces influence human osteoblast behavior during osteogenesis and the initial phases of bone deposition. Human osteoblasts from human adipose stem cells were sorted by flow cytometric analysis and induced to differentiate. After 40 days, the osteogenic differentiation was detected by alizarin red staining, and the alkaline phosphatase (ALP) was evaluated with western blot (WB) and real-time reverse transcriptase-polymerase chain reaction (RT-PcR) analysis. After confluence, human osteoblasts were cultured onto two different innovative laser-obtained titanium surfaces (L1 and L2) and compared with one sandblasted and acid-etched (SBAE) surface as the control. At different times, human osteoblast behavior was evaluated with cell proliferation viability assay (MTT), scanning electron microscopy (SEM), energy-dispersive x-rays (EDAX), osteogenic markers with RT-PcR, and WB analysis of matrix extracellular phosphoglycoprotein (MEPE), ALP, and osteocalcin (OCN). After cell sorting, the human osteoblasts from human adipose stem cells showed increasing values of ALP mRNA and protein expression. The osteogenic differentiati...Continue Reading

Citations

Jan 23, 2021·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Bao-Ming TangZhuo-Ya Wang
Jan 28, 2021·Clinical Oral Implants Research·Jefferson O AbariciaRene Olivares-Navarrete

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