PMID: 9188612Jul 1, 1997Paper

Effects of nucleotide changes on the ability of hepatitis delta virus to transcribe, process, and accumulate unit-length, circular RNA

Journal of Virology
T T WuJ Taylor

Abstract

The circular RNA genome of hepatitis delta virus (HDV) can fold into an unbranched rodlike structure. We mutagenized the two ends of this structure and assayed the effects on the ability of the genomes to replicate and accumulate processed RNA transcripts in transfected cells. The top end, defined as that nearest to the 5' end of the putative mRNA for delta antigen, was much more sensitive than the other end, defined as the bottom. Most of the 22 mutants made at the bottom were able to accumulate RNA as well as the wild type. For deletions extending as close as 2 nucleotides (nt) from the predicted domains needed for the two ribozymes, the accumulation levels dropped to <0.1%. In one mutant, 13 nt of HDV was replaced with 57 nt of non-HDV sequences, and accumulation was at 20% of the wild-type level, consistent with the potential of HDV to act as a vector. However, after replacement with a second sequence, accumulation dropped to 1%. For most of the 14 mutants made at the top of the rod, we observed dramatic inhibitory effects. For example, after removal of 3 bp from the stem adjacent to the terminal loop, accumulation dropped to <0.06% of the wild-type genome level. The top region that we considered was adjacent to both the 5'...Continue Reading

References

Aug 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J L CaseyJ L Gerin
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·P J ChenJ Taylor
Aug 1, 1988·Journal of Virology·L SharmeenJ Taylor
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Nov 1, 1995·Journal of Virology·T T WuJ M Taylor
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Oct 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·J L CaseyJ L Gerin
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May 1, 1996·Journal of Virology·V V BichkoJ M Taylor

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Citations

May 24, 2000·Journal of Virology·G MoraledaJ Taylor
Oct 29, 2003·Journal of Virology·Jinhong ChangJohn M Taylor
Apr 14, 2004·Journal of Virology·Xingcao NieJohn M Taylor
Mar 3, 2004·Journal of Virology·Sheng-Chieh HsuWan-Jr Syu
Jul 25, 2000·Journal of Virology·S GudimaJ Taylor
Sep 12, 2000·Journal of Virology·J ChangJ Taylor
May 4, 2020·Antiviral Research·Julie Lucifora, Marion Delphin
Apr 19, 2006·RNA·Severin O GudimaJohn M Taylor

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