PMID: 8955316Dec 1, 1996Paper

Effects of nutrition and growth rate on Lrp levels in Escherichia coli

Journal of Bacteriology
J R LandgrafJ M Calvo

Abstract

Lrp (leucine-responsive regulatory protein) activates some Escherichia coli operons that function in anabolism and represses others involved in catabolism (for a review, see J. M. Calvo and R. G. Matthews, Microbiol. Rev. 58:466-490, 1994). This overall pattern suggests that Lrp may help cells adapt to changes in the nutritional environment. Here, we tested the idea that the nutritional richness of the medium determines the amount of Lrp in cells. Lrp was measured directly by Western blotting (immunoblotting) in cells grown in a chemically defined rich medium or in a minimal medium. In addition, transcription from the lrp promoter was assessed with a lacZ reporter gene. The results with these two different measurements were nearly the same, indicating that under the conditions employed, beta-galactosidase measurements can accurately reflect Lrp levels. For cells in a minimal medium, Lrp levels were consistently lowest during the logarithmic phase of growth, but overall, there was not much variation in levels as a function of growth phase (1.3-fold difference between highest and lowest values). However, for cells in a rich medium, Lrp levels dropped 3- to 4-fold during the lag phase, remained constant during the log phase, and t...Continue Reading

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Citations

Dec 2, 2000·Microbiology·M F AnjumJ R Guest
Mar 8, 2002·Omics : a Journal of Integrative Biology·Denise M Wolf, Adam P Arkin
Mar 22, 2007·The Journal of Biological Chemistry·Jingshan RenRaymond J Owens
Dec 5, 2008·Proceedings of the National Academy of Sciences of the United States of America·Byung-Kwan ChoBernhard Ø Palsson
May 1, 2010·Journal of Cellular Physiology·Jun WuJianping Xie
Aug 12, 2016·Nature Reviews. Microbiology·Megan BergkesselDianne K Newman
May 6, 2003·Annual Review of Microbiology·Larry Reitzer
Dec 15, 2010·Archaea : an International Microbiological Journal·Eveline Peeters, Daniel Charlier
Dec 21, 2010·Journal of Bacteriology·Benjamin R Hart, Robert M Blumenthal
May 17, 2011·FEMS Microbiology Reviews·Ding Jun JinYan Ning Zhou
Nov 19, 2011·FEMS Microbiology Letters·Angelina CordoneEzio Ricca
Sep 28, 2004·Biotechnology and Bioengineering·Laura R JarboeJames C Liao
May 29, 2001·The Journal of Biological Chemistry·A MajumderH Y Wu
Oct 11, 2002·Proceedings of the National Academy of Sciences of the United States of America·Travis H TaniRowena G Matthews
Sep 27, 2018·Frontiers in Microbiology·Peter N CiacciaDhruba K Chattoraj
Jun 10, 2004·Molecular Microbiology·Javier Pizarro-Cerdá, Karsten Tedin
Feb 2, 2008·Biological & Pharmaceutical Bulletin·Tsuyoshi KawashimaMasashi Suzuki
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Mar 20, 2010·FEMS Microbiology Reviews·Juana María Navarro LlorensEsteban Martínez-García
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