Effects of obatoclax combined with gemcitabine on the biological activity of pancreatic cancer cells under hypoxic conditions

Molecular Medicine Reports
Xin-Fang HouJu-Feng Wang

Abstract

The present study aimed to investigate the effects of obatoclax (OBX) combined with gemcitabine (GEM) treatment on the proliferation, migration, invasion and epithelial‑mesenchymal transition (EMT) related proteins of pancreatic cancer cell line BxPC‑3 under hypoxic conditions. Protein expression levels of hypoxia‑inducible factor 1α (HIF‑1α) in BxPC‑3 pancreatic cancer cells under normoxic and hypoxic conditions were detected by western blotting. Cells were divided into four groups: Normoxia group, hypoxia group, OBX group and OBX + GEM group. The proliferation activity of BxPC‑3 cells was detected by Cell Counting kit‑8. The migratory and invasive abilities of BxPC‑3 cells were detected by the scratch test and Matrigel assay, respectively. The protein expression levels of vimentin, E‑cadherin and p53 in BxPC‑3 cells were also detected by western blotting. HIF‑1α expression under hypoxic conditions was significantly increased compared with expression under normoxic conditions. Under hypoxic conditions, OBX treatment reduced cell activity, decreased cell migration and invasion, promoted the expression of E‑cadherin and p53. In the OBX + GEM group, BxPC‑3 cell activity decreased significantly, cell migration and invasion decreas...Continue Reading

References

Nov 10, 2000·International Journal of Radiation Oncology, Biology, Physics·A C KoongM Vierra
Mar 29, 2002·Medical Oncology·P VaupelM Hoeckel
Feb 7, 2008·PloS One·Tamar GeigerAlexander Levitzki
Jul 9, 2008·Translational Oncology·Meredith A MorganTheodore S Lawrence
Oct 16, 2008·Anti-cancer Agents in Medicinal Chemistry·Jean-Philippe Cosse, Carine Michiels
Jan 2, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Shengbing HuangFrank A Sinicrope
Feb 18, 2010·Liver International : Official Journal of the International Association for the Study of the Liver·Bryan L Copple
Mar 12, 2011·The Journal of Clinical Investigation·Luke R E HarrisonCaroline Dive
Apr 27, 2011·The Journal of Experimental Medicine·Taewan KimCarlo M Croce
Jun 4, 2011·Cellular and Molecular Life Sciences : CMLS·Arun Satelli, Shulin Li
Jan 10, 2012·Breast Cancer Research : BCR·Luciana P SchwabTiffany N Seagroves
Mar 9, 2012·Trends in Pharmacological Sciences·Gregg L Semenza
Feb 15, 2013·Proceedings of the National Academy of Sciences of the United States of America·Fabienne GuillaumondSophie Vasseur
Mar 26, 2013·Journal of Cell Science·Marta CanelValerie G Brunton
Jul 17, 2013·CA: a Cancer Journal for Clinicians·Christopher L WolfgangRalph H Hruban
Dec 4, 2013·The Journal of Cell Biology·Crystal D RogersMarianne E Bronner
Mar 8, 2014·World Journal of Gastroenterology : WJG·Margaret G KeaneGiuseppe K Fusai
Sep 11, 2014·The New England Journal of Medicine·David P RyanNabeel Bardeesy
Jul 18, 2015·World Journal of Gastroenterology : WJG·Quan-Jun LinDe-Liang Fu
Jan 9, 2016·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal
Mar 5, 2016·Postȩpy higieny i medycyny doświadczalnej·Barbara Borowa-Mazgaj
May 18, 2016·International Journal of Molecular Sciences·Paola BendinelliMaria Alfonsina Desiderio
Apr 5, 2017·Chinese Journal of Cancer Research = Chung-kuo Yen Cheng Yen Chiu·Wen ZhengJun Zhu

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