Effects of octenidine applied alone or mixed with sodium hypochlorite on eukaryotic cells

International Endodontic Journal
H Coaguila-LlerenaG Faria

Abstract

To assess the effects of octenidine dihydrochloride (OCT) on eukaryotic cells and the cytotoxicity of OCT associated with sodium hypochlorite - NaOCl (NaOCl/OCT). L929 fibroblasts and human osteoblast-like cells (Saos-2) were exposed to 0.1% OCT, 2% CHX, 2.5% NaOCl, 5.25% NaOCl and mixtures of 5.25% NaOCl and 0.1% OCT (NaOCl/OCT) at 90 : 10, 80 : 20 and 50 : 50 ratios. Cell viability was assessed by methyl-thiazol-tetrazolium (MTT) and neutral red (NR) assays; type of cell death, by flow cytometry; cytoskeleton, by actin and α-tubulin fluorescence; and alkaline phosphatase (ALP) activity, by thymolphthalein release. The data were analysed by two-way ANOVA and Bonferroni tests (α = 0.05). MTT and NR assays revealed that 0.1% OCT had the lowest cytotoxicity (P < 0.05), followed by 2% CHX (P < 0.05). The 2.5% NaOCl, NaOCl/OCT 80 : 20 and NaOCl/OCT 50 : 50 solutions had intermediate cytotoxicity. NaOCl 5.25% and NaOCl/OCT 90 : 10 had the highest cytotoxicity (P < 0.05). The OCT group had a higher percentage of viable cells than the NaOCl and CHX groups (P < 0.05), and induced apoptosis at higher doses. The cytoskeleton alterations were observed at 0.12%, 0.6% and 2.02% for the NaOCl, CHX and OCT groups, respectively. The solutions ...Continue Reading

References

Dec 1, 1973·Oral Surgery, Oral Medicine, and Oral Pathology·L SpangbergK Langeland
Apr 20, 2001·International Endodontic Journal·H K HaapasaloM P Haapasalo
Apr 15, 2003·Clinical Microbiology Reviews·Peter Gilbert, Andrew J McBain
Jan 14, 2004·International Endodontic Journal·L A OkinoJ A P Figueiredo
Apr 19, 2006·Journal of Neuroscience Research·Peishan Liu-SnyderRichard Ben Borgens
Jun 7, 2006·Free Radical Research·Yoshiro SaitoEtsuo Niki
Oct 28, 2008·Biochimica Et Biophysica Acta·Katsumi Matsuzaki
Nov 26, 2008·Toxicology and Applied Pharmacology·Gisele FariaMarcos A Rossi
Jul 21, 2009·Toxicological Sciences : an Official Journal of the Society of Toxicology·Thomas Hartung, George Daston
Jan 30, 2010·Nature·Daniel A Fletcher, R Dyche Mullins
Feb 2, 2010·Journal of Endodontics·Yoshiyuki YasudaTakashi Saito
Jul 17, 2012·Journal of Endodontics·Mahmoud Reza ModareszadehNarges Salamat
Nov 20, 2013·International Endodontic Journal·M YadlapatiR M Silva
May 31, 2015·Journal of Endodontics·Daiana Elisabeth BöttcherFabiana Soares Grecca
Jun 16, 2015·Journal of Wound Care·S JenullM Huettinger
Oct 3, 2015·European Journal of Dentistry·Dilara ArslanSeyda Herguner Siso
Nov 12, 2015·Clinical Oral Investigations·Alexander WelkGerald Müller
Dec 15, 2015·International Endodontic Journal·M Ruiz-LinaresC M Ferrer-Luque
Apr 10, 2016·Archives of Oral Biology·Maurilio MalavasiMarcelo Henrique Napimoga
Jan 31, 2017·International Endodontic Journal·K S ViolaG Faria
Aug 22, 2017·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·R Alvarez-MarinL Poirel
Jun 6, 2019·Brazilian Dental Journal·Hernán Coaguila-LlerenaGisele Faria

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis