PMID: 20650069Apr 1, 1995Paper

Effects of ofloxacin on integrin expression on epiphyseal mouse chondrocytes in vitro

Toxicology in Vitro : an International Journal Published in Association with BIBRA
Mehdi ShakibaeiRalf Stahlmann

Abstract

Quinolone-induced arthropathy is an important toxic effect in immature animals that has led to restrictions of the therapeutic use of these antimicrobial agents. The effects of ofloxacin on epiphyseal chondrocytes from 17-day-old mouse foetuses were studied in vitro. Adhesion of the cells to culture dishes was impaired in a concentration-dependent manner and was first perceptible at a concentration of 10 mg ofloxacin/litre medium. A closer analysis by immunomorphological methods showed that the expression of several integrin receptors (beta1, alpha3, alpha5beta1, alphavbeta3) on the chondrocytes was reduced. Again, first alterations occurred at the rather low concentration of 10 mg ofloxacin/litre medium, and at 30 mg ofloxacin/litre medium alpha3- and alpha5beta1 integrins were demonstrable on 50% or less of the cultured cells. Based on these findings in vitro, a new hypothesis for the mechanism of the chondrotoxicity of quinolones is proposed: the ability of these antimicrobials to form chelate complexes with divalent cations could explain why the integrin receptors on chondrocytes are altered after quinolone exposure, since it is well known that the function of the integrin receptor depends on calcium or magnesium ions. Furt...Continue Reading

References

Jul 15, 1977·International Journal of Cancer. Journal International Du Cancer·E Engvall, E Ruoslahti
Jan 1, 1992·Toxicologic Pathology·A W GoughV Baragi
Apr 1, 1992·British Journal of Rheumatology·D M SalterD L Gardner
Oct 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·G PalùM Palumbo
Dec 1, 1992·The Pediatric Infectious Disease Journal·U B Schaad
Oct 1, 1992·The Journal of Antimicrobial Chemotherapy·U B Schaad, J Wedgwood
Jul 1, 1992·Drug Safety : an International Journal of Medical Toxicology and Drug Experience·X ChevalierB Larget-Piet
Jan 1, 1992·Annual Review of Medicine·H C Neu
Dec 1, 1991·Matrix : Collagen and Related Research·C Schröter-KermaniH J Merker
Jan 1, 1991·The Journal of Clinical Investigation·E Ruoslahti
Dec 2, 1990·Cell Differentiation and Development : the Official Journal of the International Society of Developmental Biologists·V Quaranta
Sep 1, 1989·Experimental Cell Research·Y SommarinD Heinegård
Sep 1, 1987·The Journal of Cell Biology·W S ArgravesE Ruoslahti
Jul 1, 1988·Fundamental and Applied Toxicology : Official Journal of the Society of Toxicology·M Kato, T Onodera
Jan 1, 1980·Virchows Archiv. A, Pathological Anatomy and Histology·K GrundmannH J Merker
Jan 1, 1993·Drugs·U B Schaad
Dec 1, 1993·Drug Safety : an International Journal of Medical Toxicology and Drug Experience·R StahlmannD Van Sickle
Feb 1, 1993·Fundamental and Applied Toxicology : Official Journal of the Society of Toxicology·J E BurkhardtW W Carlton
Dec 1, 1992·International Journal of Antimicrobial Agents·R A Robson
Sep 1, 1992·Toxicology in Vitro : an International Journal Published in Association with BIBRA·C Schröter-KermaniH J Merker
Jul 1, 1994·Trends in Cardiovascular Medicine·H S Baldwin, C A Buck

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Citations

Dec 1, 1996·Toxicology in Vitro : an International Journal Published in Association with BIBRA·G KempkaG Schlüter
May 4, 2013·Cellular and Molecular Life Sciences : CMLS·Toshimi Michigami
Nov 29, 2013·Arthritis Research & Therapy·Constanze BuhrmannMehdi Shakibaei
Dec 3, 1999·Antimicrobial Agents and Chemotherapy·M A SimoninB Terlain

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