Effects of organic solvents on the activity of free and immobilised laccase from Rhus vernicifera

International Journal of Biological Macromolecules
Yun-Yang WanJohn F Kennedy

Abstract

Rhus laccase (RL) was covalently immobilised onto chitosan, and the effects of immobilisation on pH optimum, enzyme activity, thermostability, and re-use evaluated, using either N,N-dimethyl-p-phenylenediamine or 2,6-dimethoxyphenol as substrate. Immobilisation greatly enhanced enzyme thermostability, resulted in negligible loss of activity, and showed excellent re-use potential, with >80% relative activity retained after 15 cycles in aqueous solvent. Immobilised Rhus laccase (I-RL) was more catalytically active in both hydrophobic and hydrophilic organic solvents than free RL. With water-immiscible organic solvents, both free RL and I-RL required a minimum water content to achieve activity. With water-miscible organic solvents, in general a water content of ∼20-50% (v/v) was required to achieve activity using free RL, whereas with I-RL less water was generally required to achieve enzyme activity, and therefore considerably higher relative activity was exhibited at lower water contents. Kinetic investigations showed that the rate of substrate disappearance generally followed a pseudo-first-order law, and for evaluated water-immiscible organic solvents rate constants generally increased with decrease of hydrophobicity, however, ...Continue Reading

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Citations

Feb 6, 2014·Applied Biochemistry and Biotechnology·Wei-Wei ZhangXiao-Qi Yu
Apr 9, 2014·Journal of Biotechnology·Stefan KoniecznyJoerg C Tiller
Dec 27, 2011·Bioresource Technology·Jesús Fernández-LucasMiguel Arroyo
May 19, 2012·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·M Gökgöz, H Altinok
Jul 3, 2016·Biotechnology and Bioengineering·Ramona PlotheJoerg C Tiller
Apr 11, 2017·Biotechnology Progress·Brandt BertrandMaría R Trejo-Hernández
May 9, 2019·Preparative Biochemistry & Biotechnology·Mahalakshmi SarathiGautam Pennathur
Sep 27, 2019·Chembiochem : a European Journal of Chemical Biology·Brandon T Cisneros, Neal K Devaraj
Jul 7, 2019·Scientific Reports·Meng-Hsuan WuTuan-Hua David Ho
Aug 10, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Adriany das Graças Nascimento AmorimBeatriz López-Ruiz

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