PMID: 16529320Mar 15, 2006Paper

Effects of osteoblasts and inductor on proliferation and osteodifferentiation of marrow stromal stem cells

Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery
Kai HuangGuofeng Cai

Abstract

To find a new culture system to induce proliferation and osteodifferentiation of marrow stromal cells (MSCs) in vitro for bone tissue engineering. There were four groups in this experiment to study effects of Passage 3 osteoblasts derived from the rat cranium and the osteogenic inductor (1 nmol/L dexamethasone, 10 mmol/L beta-glycero-phosphate, 50 microg/ml retinoic acid) on growth of MSCs isolated from the rat femur and the tibia. MSCs were cultured in the DMEM medium (the control group) and in the osteoinductive culture medium (the inductor group); furthermore, MSCs were co-cultured with the osteoblasts in the DMEM medium (the osteoblast group) and in the osteoinductive culture medium (the combined treatment group). The cells in the four groups were counted every 2 days for 8 days and alkaline phosphatase (ALP) activity of MSCs at 10 days of cultivation was measured. The MRNA expression of osteocalcin (OC) of MSCs at 2 weeks was assayed with the reverse transcript polymerase chain reaction (RT-PCR). There were more cells in the osteoblast group than in the control group (31.73 +/- 3.31 x 10(4) VS. 24.33 +/- 3.04 x 10(4), P < 0. 05), but there were fewer cells in the inductor group (16.23 +/- 2.44 x 10(4), P < 0.05). There was...Continue Reading

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