PMID: 3763371Sep 1, 1986Paper

Effects of ouabain and temperature on cell membrane potentials in isolated perfused straight proximal tubules of the mouse kidney

Pflügers Archiv : European journal of physiology
H VölklF Lang

Abstract

In isolated perfused segments of the mouse proximal tubule, the potential difference across the basolateral cell membrane (PDbl) was determined with conventional microelectrodes. Under control conditions with symmetrical solutions it amounted to -62 +/- 1 mV (n = 118). The potential difference across the epithelium (PDte) was -1.7 +/- 0.1 mV (n = 45). Transepithelial resistance amounted to 1.82 +/- 0.09 k omega cm (n = 28), corresponding to 11.4 +/- 0.6 omega cm2. Increasing bath potassium concentration from 5 to 20 mmol/l depolarized PDbl by +24 +/- 1 mV (n = 103), and PDte by +1.6 +/- 0.1 mV (n = 19). Thus, the basolateral cell membrane is preferably conductive to potassium. Rapid cooling of the bath perfusate from 38 degrees C to 10 degrees C led to a transient hyperpolarization of PDbl from -60 +/- 1 to -65 +/- 1 mV (n = 21) within 40 s followed by gradual depolarization by +18 +/- 1% (n = 14) within 5 min. The transepithelial resistance increased significantly from 1.78 +/- 0.11 k omega cm to 2.20 +/- 0.21 k omega cm (n = 15). Rapid rewarming of the bath to 38 degrees C caused a depolarization from -61 +/- 2 mV (n = 17) to -43 +/- 2 mV (n = 16) within 15 s followed by a repolarization to -59 +/- 2 mV (n = 10) within 40 s. ...Continue Reading

References

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Citations

Jan 1, 1995·Pflügers Archiv : European journal of physiology·R KletaE Schlatter
Oct 1, 1987·Pflügers Archiv : European journal of physiology·H Gögelein, R Greger
May 1, 1988·Pflügers Archiv : European journal of physiology·H Völkl, F Lang
Mar 1, 1988·Pflügers Archiv : European journal of physiology·A Schwab, H Oberleithner
Aug 1, 1990·The Journal of Membrane Biology·H Völkl, F Lang
Apr 1, 1989·The Journal of Membrane Biology·B A Vance, B A Biagi
Dec 8, 1988·Biochimica Et Biophysica Acta·H Völkl, F Lang
Dec 12, 1991·Biochimica Et Biophysica Acta·D Häussinger, F Lang
Apr 14, 2010·Proceedings of the National Academy of Sciences of the United States of America·Shigeaki MutoMikio Furuse
Apr 26, 2013·Kidney International·Giovambattista CapassoJohn P Geibel
Dec 1, 1991·Kidney International·H Völkl, F Lang
May 12, 2010·European Journal of Clinical Investigation·Michael FöllerFlorian Lang
Aug 30, 2014·Biochemical and Biophysical Research Communications·Zohreh HosseinzadehFlorian Lang
Jan 26, 2017·Scientific Reports·Zohreh HosseinzadehFlorian Lang
Oct 28, 2016·American Journal of Physiology. Renal Physiology·Shigeaki Muto
Dec 7, 2013·American Journal of Physiology. Cell Physiology·Shefalee K BhavsarFlorian Lang
Jul 1, 1988·Pflügers Archiv : European journal of physiology·H Völkl, F Lang
May 12, 2000·American Journal of Physiology. Renal Physiology·M TraebertB Kaissling
Jun 1, 1987·Pflügers Archiv : European journal of physiology·H GögeleinE Schlatter
Oct 1, 1995·Kidney International·J Hirsch, E Schlatter

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