PMID: 9640666Jun 26, 1998Paper

Effects of over-expression of strictosidine synthase and tryptophan decarboxylase on alkaloid production by cell cultures of Catharanthus roseus

Planta
C CanelRobert Verpoorte

Abstract

Cells of Catharanthus roseus (L.) G. Don were genetically engineered to over-express the enzymes strictosidine synthase (STR; EC 4.3.3.2) and tryptophan decarboxylase (TDC; EC 4.1.1.28), which catalyze key steps in the biosynthesis of terpenoid indole alkaloids (TIAs). The cultures established after Agrobacterium-mediated transformation showed wide phenotypic diversity, reflecting the complexity of the biosynthetic pathway. Cultures transgenic for Str consistently showed tenfold higher STR activity than wild-type cultures, which favored biosynthetic activity through the pathway. Two such lines accumulated over 200 mg.L-1 of the glucoalkaloid strictosidine and/or strictosidine-derived TIAs, including ajmalicine, catharanthine, serpentine, and tabersonine, while maintaining wild-type levels of TDC activity. Alkaloid accumulation by highly productive transgenic lines showed considerable instability and was strongly influenced by culture conditions, such as the hormonal composition of the medium and the availability of precursors. High transgene-encoded TDC activity was not only unnecessary for increased productivity, but also detrimental to the normal growth of the cultures. In contrast, high STR activity was tolerated by the cult...Continue Reading

Citations

May 19, 2011·Applied Microbiology and Biotechnology·Mei-Liang ZhouYi-Xiong Tang
Jul 5, 2007·Plant Cell Reports·Sonia Gómez-GaleraPaul Christou
Apr 21, 2007·Science in China. Series C, Life Sciences·MaoJun Xu, JuFang Dong
Jan 1, 2009·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Toolika SrivastavaP S Srivastava
Apr 16, 2002·Current Opinion in Biotechnology·R Verpoorte, J Memelink
Apr 20, 2001·Current Opinion in Plant Biology·V De Luca, P Laflamme
May 5, 2001·Annual Review of Plant Physiology and Plant Molecular Biology·Peter J Facchini
Feb 7, 2008·Annual Review of Plant Biology·Jörg Ziegler, Peter J Facchini
Jan 8, 2013·Bioscience, Biotechnology, and Biochemistry·Fumihiko Sato
Apr 2, 2013·Annual Review of Chemical and Biomolecular Engineering·Jong Moon YoonJacqueline V Shanks
Apr 21, 2009·Nature Chemical Biology·Effendi LeonardKristala Jones Prather
May 15, 2008·The Plant Journal : for Cell and Molecular Biology·Peter J Facchini, Vincenzo De Luca
Aug 29, 2013·Plant Biotechnology Journal·Joseph G DubouzetKyo Wakasa
Aug 28, 2004·Plant Physiology and Biochemistry : PPB·Felipe Vázquez-FlotaMaría De Lourdes Miranda-Ham
Oct 20, 2006·Biomolecular Engineering·Mattijs K JulsingOliver Kayser
Jan 22, 2002·Metabolic Engineering·Erik H Hughes, Jacqueline V Shanks
Oct 6, 2016·Critical Reviews in Biotechnology·Ellis C O'Neill, Steven Kelly
Apr 11, 2000·The Journal of Pharmacy and Pharmacology·R Verpoorte
Feb 17, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Lorena AlmagroMaria Angeles Pedreño

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biosynthetic Transformations

Biosyntheic transformtions are multi-step, enzyme-catalyzed processes where substrates are converted into more complex products in living organisms. Simple compounds are modified, converted into other compounds, or joined together to form macromolecules. Discover the latest research on biosynthetic transformations here.