Effects of overexpression of endogenous phenylalanine ammonia-lyase (PALrs1) on accumulation of salidroside in Rhodiola sachalinensis.

Plant Biology
L-Q MaHong Wang

Abstract

Salidroside, a novel effective adaptogenic drug extracted from the medicinal plant Rhodiola sachalinensis A. Bor, can be derived from phenylalanine or tyrosine. Due to the scarcity of R. sachalinensis and its low yield of salidroside, there is great interest in enhancing production of salidroside by the plant. In this study, a cDNA clone encoding phenylalanine ammonia-lyase (PAL) was isolated from R. sachalinensis using rapid amplification of cDNA ends. The resulting cDNA was designated PALrs1. It is 2407-bp long and encodes 710 deduced amino acid residues. Southern blot analysis of genomic DNA indicated that the PAL gene family is composed of three to five genes in the R. sachalinensis genome. Northern blot analysis revealed that transcripts of PALrs1 were present in calli, leaves and stems, but expression in roots was very low. The PALrs1 under the 35S promoter with double-enhancer sequences from CaMV-Omega and TMV-Omega fragments was transferred into R. sachalinensis via Agrobacterium tumefaciens. PCR and PCR-Southern blot confirmed that the PALrs1 gene had been integrated into the genome of transgenic plants. Northern blot analysis revealed that the PALrs1 gene had been expressed at the transcriptional level. High-performan...Continue Reading

References

Jan 1, 1997·Plant Physiology·J RöslerJ Schmid
Nov 26, 2003·Journal of Biotechnology·Shuangxiu WuMadeline Wu
Apr 5, 2005·The Plant Cell·Julian C VerdonkRobert C Schuurink
Sep 8, 2006·Phytochemistry·Gad G YousefMary Ann Lila

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Citations

Nov 12, 2013·Protein Expression and Purification·Kai Zhang, Ye Ni
Jul 28, 2015·Phytochemistry Reviews : Proceedings of the Phytochemical Society of Europe·Marta Grech-BaranAgnieszka Pietrosiuk
Jun 15, 2016·Scientific Reports·Jian-Xiong ZhangZhi-Dan Zhao
Aug 10, 2018·Applied Biochemistry and Biotechnology·Sahil KapoorOm Prakash Chaurasia
Feb 27, 2018·Phytochemistry·Craig A Schenck, Hiroshi A Maeda
Jul 1, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Fangfang FanXiaobo Wang

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