Effects of overexpression of the Na+-Ca2+ exchanger on [Ca2+]i transients in murine ventricular myocytes

Circulation Research
A YaoW H Barry

Abstract

We measured [Ca2+]i and [Na+]i in isolated transgenic (TG) mouse myocytes overexpressing the Na+-Ca2+ exchanger and in wild-type (WT) myocytes. In TG myocytes, the peak systolic level and amplitude of electrically stimulated (ES) [Ca2+]i transients (0.25 Hz) were not significantly different from those in WT myocytes, but the time to peak [Ca2+]i was significantly prolonged. The decline of ES [Ca2+]i transients was significantly accelerated in TG myocytes. The decline of a long-duration (4-s) caffeine-induced [Ca2+]i transient was markedly faster in TG myocytes, and [Na+]i was identical in TG and WT myocytes, indicating that the overexpressed Na+-Ca2+ exchanger is functionally active. The decline of a short-duration (100-ms) caffeine-induced [Ca2+]i transient in 0 Na+/0 Ca2+ solution did not differ between the two groups, suggesting that the sarcoplasmic reticulum (SR) Ca2+-ATPase function is not altered by overexpression of the Na+-Ca2+ exchanger. There was no difference in L-type Ca2+ current density in WT and TG myocytes. However, the sensitivity of ES [Ca2+]i transients to nifedipine was reduced in TG myocytes. This maintenance of [Ca2+]i transients in nifedipine was inhibited by Ni2+ and required SR Ca2+ content, consistent...Continue Reading

References

Oct 1, 1992·Cardiovascular Research·G Callewaert
Apr 1, 1989·The American Journal of Physiology·M J Shattock, D M Bers
Feb 1, 1989·The American Journal of Physiology·K W Spitzer, J H Bridge
May 1, 1988·Journal of Molecular and Cellular Cardiology·D M BersT X Nguyen
Jan 3, 1995·Proceedings of the National Academy of Sciences of the United States of America·J S ShamM Morad
Jan 1, 1995·The American Journal of Physiology·D M Bers, J R Berlin
Mar 1, 1994·Journal of Cardiovascular Electrophysiology·A J LeviP Brooksby
Feb 1, 1994·The Journal of Physiology·P Lipp, E Niggli
Sep 15, 1994·Biochemical and Biophysical Research Communications·H TeradaN Yamazaki
Apr 1, 1993·Pflügers Archiv : European journal of physiology·A VarroD A Eisner
Jan 1, 1997·The Journal of General Physiology·O S Andersen
Jul 1, 1997·Biophysical Journal·J A CopelloS Fleischer

❮ Previous
Next ❯

Citations

Apr 2, 2005·Methods in Cell Science : an Official Journal of the Society for in Vitro Biology·David M Plank, Mark A Sussman
Jun 28, 2005·Progress in Biophysics and Molecular Biology·Andrew F JamesJules C Hancox
May 10, 2005·Journal of Molecular and Cellular Cardiology·Loren E WoldAmy J Davidoff
May 24, 2000·European Journal of Pharmacology·M IzumiH Karaki
Mar 8, 2003·Journal of Molecular and Cellular Cardiology·Fons VerdonckKarin R Sipido
Jun 24, 2003·Journal of Molecular and Cellular Cardiology·Zhi SuWilliam H Barry
May 28, 2003·Cell Calcium·Michael RitterWilliam H Barry
May 22, 2001·International Immunopharmacology·M RitterW H Barry
Aug 3, 2005·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·David M Plank, Mark A Sussman
Feb 7, 2001·The Journal of General Physiology·C R WeberD M Bers
Jan 15, 2000·Journal of Cardiovascular Electrophysiology·J JalifeD Vaidya
Jun 9, 2000·Annual Review of Physiology·K D Philipson, D A Nicoll
Jun 9, 2000·Annual Review of Physiology·H Kiriazis, E G Kranias
Jun 16, 2009·American Journal of Physiology. Heart and Circulatory Physiology·JuFang WangJoseph Y Cheung
Oct 27, 2009·American Journal of Physiology. Heart and Circulatory Physiology·William H BarryThomas P Kennedy
Jun 6, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Amy L TuckerJoseph Y Cheung
May 8, 2003·Biological Procedures Online·Nicholas S. Aberle II, Jun Ren
Dec 22, 1999·The Japanese Journal of Physiology·H IshidaH Nakazawa
Aug 6, 2014·Proceedings of the National Academy of Sciences of the United States of America·Ang GuoLong-Sheng Song
Apr 11, 2000·Journal of Molecular and Cellular Cardiology·M RitterW H Barry
May 7, 2002·Journal of Molecular and Cellular Cardiology·Donald M Bers
May 7, 2002·Journal of Molecular and Cellular Cardiology·Hardeep K RanuSian E Harding
Feb 22, 2001·Journal of Molecular and Cellular Cardiology·L B StullC Schomisch Moravec
Sep 1, 2000·Journal of Molecular and Cellular Cardiology·S R HouserJ Weisser
Apr 18, 2009·Toxicology and Applied Pharmacology·Shigeyuki YamadaWilliam H Barry
Mar 21, 2008·Journal of Molecular and Cellular Cardiology·Chang-Soo HongDo Han Kim
Aug 4, 2007·Progress in Neurobiology·Tamás L Török
Jun 19, 2007·Journal of Molecular and Cellular Cardiology·Halvor K MørkWilliam E Louch
May 23, 2007·Journal of Cardiac Failure·Kenneth P RoosKenneth D Philipson
Mar 31, 2007·Journal of Cardiac Failure·Julieta PalomequeAlicia Mattiazzi
Aug 12, 2005·Annals of the New York Academy of Sciences·Joshua I GoldhaberKenneth D Philipson
Jun 18, 2004·Annals of the New York Academy of Sciences·Martin Morad, My Chau

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cardiac Electrophysiology

Cardiac electrophysiology is the study of electrical activities of the heart and includes the assessment, diagnosis, and treatment of cardiac events. Find the latest research on cardiac electrophysiology here.