Effects of oxidation of human parathyroid hormone on its biological activity in continuously infused, thyroparathyroidectomized rats

Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
N Horiuchi

Abstract

The effect of oxidation of human parathyroid hormone 1-34 (hPTH 1-34) on the hormone's biological activity was assessed in vivo using a multiparameter, thyroparathyroidectomized (TPTX) rat model. The peptide was oxidized by treatment with hydrogen peroxide, and the oxidized form (8,18-methionine sulfoxide) was isolated by reverse-phase HPLC. Vitamin D-deficient rats were infused with either intact or oxidized hormone along with a 5 mM calcium chloride solution for 4 or 18 hr. Infusion of nonoxidized hormone (0.1-0.8 nmoles/hr) resulted in dose-dependent increases in serum calcium, decreases in serum phosphate, decreases in urine calcium, increases in urine phosphate and cAMP, and increased renal 1,25-dihydroxyvitamin D3 (1,25 (OH)2D3) production. Oxidized PTH infused at doses up to 0.8 nmole/hr had no effect on any of these parameters. To assess the effect of oxidation on the ability of PTH to inhibit the production of the 24,25-dihydroxyvitamin D3 (24,25(OH)2D3), the infusion protocol was performed in vitamin D-deficient rats repleted with 1,25(OH)2D3 by injection. In these experiments, intact hormone markedly suppressed 24,25(OH)2D3 production, whereas the oxidized form was without effect. We conclude that intact methionine r...Continue Reading

References

Feb 1, 1986·Archives of Biochemistry and Biophysics·A L Frelinger, J E Zull
May 1, 1985·Proceedings of the Society for Experimental Biology and Medicine·S C Miller, A D Kenny
Nov 1, 1982·Proceedings of the Society for Experimental Biology and Medicine·A D Kenny, P K Pang
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER
May 1, 1950·Proceedings of the Society for Experimental Biology and Medicine·R S HARE

❮ Previous
Next ❯

Citations

Dec 3, 2016·Biochemical and Biophysical Research Communications·Juan A ArduraPeter A Friedman
Mar 7, 2018·Clinical Journal of the American Society of Nephrology : CJASN·Sarah Seiler-MusslerGunnar H Heine
Oct 22, 2019·Seminars in Dialysis·Etienne CavalierPierre Delanaye
Aug 16, 2017·Clinical Chemistry and Laboratory Medicine : CCLM·Stan R UrsemAnnemieke C Heijboer
Mar 18, 2020·Clinica Chimica Acta; International Journal of Clinical Chemistry·Stan R UrsemAnnemieke C Heijboer
Apr 25, 2021·Kidney International·Tilman B Drüeke, Jürgen Floege

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.