PMID: 6978298Dec 1, 1981Paper

Effects of oxygen and sulphydryl-containing compounds on irradiated transforming DNA. Part I. Actions of dithiothreitol

International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine
K D HeldB D Michael

Abstract

The actions and interactions of oxygen and the sulphydryl-containing compound dithiothreitol (DTT) upon the radiation sensitivity of the biological activity of purified Bacillus subtilis transforming DNA have been examined. It has previously been shown that the sensitivity of transforming DNA irradiated in dilute solution is less when irradiation is performed in 100 per cent O2 than when in 100 per cent N2, i.e. O2 protects transforming DNA with a dose-modifying factor of about 0.7. DTT protects transforming DNA in a manner that is dependent on DTT concentration and on gassing conditions. In O2 the DTT protection can largely be attributed to the scavenging of .OH radicals by the DTT, but in anoxia DTT exerts a further protective effect which results in an increasing oxygen enhancement ratio (o.e.r.) with increasing DDT concentration to a maximum o.e.r. of about 14 at 2-5 mM DTT. This additional protective effect of DTT is attributable to hydrogen atom donation from DTT to DNA radicals, thus chemically repairing the DNA. Oxygen appears to block this chemical repair reaction.

Citations

May 26, 2017·Sensors·Søren FjelstrupBirgitta Ruth Knudsen
Jul 1, 1995·The International Journal of Biochemistry & Cell Biology·R SharmaH M Mehendale
Jan 1, 1988·Pharmacology & Therapeutics·E RiklisM Mintsberg

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.