Effects of oxygen concentration on the intermediary metabolism of Leishmania major promastigotes

Molecular and Biochemical Parasitology
F P Keegan, J J Blum

Abstract

Leishmania major promastigotes grown in late log phase were incubated with glucose as sole exogenous carbon source in the presence of 5% CO2 and the amounts of glucose consumed and of the major products formed--succinate, pyruvate, alanine, acetate, glycerol, and D-lactate--were measured as a function of pO2. Glucose consumption increased as pO2 was lowered to 6% (a positive Pasteur effect) and then declined to the same level at 95% N2 as at 95% O2. The production of D-lactate and of glycerol increased as pO2 dropped from 95%, reaching a maximum at about 2% O2. Succinate production, however, increased dramatically when pO2 was reduced to 6% and remained at that level with further reduction of pO2. The amount of succinate produced relative to the amount of glucose carbon consumed suggests utilization of an endogenous carbon source. Acetate production did not change between 95% O2 and 6% O2 and then declined with decreasing pO2. These observations suggest the presence of two sensors, one with a high and one with a low affinity for oxygen. When glycerol or alanine were the only exogenous sources of carbon, the primary products released were acetate and succinate. Acetate production from alanine declined slightly as pO2 was reduced...Continue Reading

References

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Citations

Jul 1, 1992·Journal of Cellular Physiology·J J Blum
Apr 1, 1994·Journal of Bioenergetics and Biomembranes·J J Blum
Jan 26, 1995·Molecular and Cellular Biochemistry·J J CastillaA Osuna
Jul 1, 1992·Molecular and Biochemical Parasitology·F P Keegan, J J Blum
Feb 1, 1992·Molecular and Biochemical Parasitology·M J Walsh, J J Blum
Apr 1, 1993·Parasitology Today·J J Blum
Jun 14, 2002·International Journal for Parasitology·Siddhartha DasKrishna Reddy
Jul 17, 1999·Parasitology Today·A G Tielens, Van Hellemond JJ
Jan 1, 1991·The Journal of protozoology·C Burrows, J J Blum
Nov 1, 1991·The Journal of protozoology·J J Blum
Sep 1, 1992·The Journal of protozoology·J J Blum
Sep 15, 2015·PloS One·Abhishek SubramanianRam Rup Sarkar
Sep 2, 2017·Scientific Reports·Abhishek Subramanian, Ram Rup Sarkar

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