Effects of oxygen on vascular patterning in Tie1/LacZ metanephric kidneys in vitro

Biochemical and Biophysical Research Communications
S LoughnaA S Woolf

Abstract

Tie1 is an endothelial lineage-specific receptor. Using Tie1/LacZ mice we previously demonstrated in situ differentiation of glomerular capillaries after transplantation of renal precursors into the neonatal nephrogenic kidney cortex. We now report studies with Tie1/LacZ metanephric kidneys explanted in vitro at a stage when Tie1/LacZ-expressing cells surround nephron precursors but glomeruli are unformed. After 4 days of serum-free organ culture in 21% O2, transgene-expressing vessels regressed. In contrast, in 3% O2, transgene was expressed between epithelial tubules by cellular masses containing poorly defined lumens. The normal branching of Tie1/LacZ-expressing vessels which occurred in vivo was absent in vitro and glomeruli forming in culture lacked capillaries. Similar observations were made in wild-type metanephroi using vascular endothelial growth factor receptor 2 (Flk1) as an endothelial marker. We speculate that the metanephros is hypoxic in vivo to permit endothelial growth but other cues must be required for construction of the microcirculation since hypoxia failed to elicit normal patterning in vitro.

References

Nov 1, 1990·Kidney International·A S WoolfL G Fine
Oct 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·T N SatoK L Audus
Jan 1, 1997·Current Opinion in Nephrology and Hypertension·A S Woolf, C M Cale
Mar 15, 1997·Developmental Biology·A Tufro-McReddieR A Gomez
Apr 2, 1998·Experimental Nephrology·A S Woolf, S Loughna

❮ Previous
Next ❯

Citations

Aug 31, 2013·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Jamie A Davies, C-Hong Chang
Oct 24, 2000·Kidney International·H T YuanA S Woolf
Apr 21, 2005·Kidney International·Barbara J Ballermann
Oct 23, 2012·Journal of the American Society of Nephrology : JASN·Christodoulos XinarisGiuseppe Remuzzi
Oct 23, 2012·Organogenesis·Aleksandra Rak-RaszewskaPatricia Murray
Mar 19, 2014·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Donna Beer Stolz, Sunder Sims-Lucas
Aug 17, 2001·Developmental Dynamics : an Official Publication of the American Association of Anatomists·M Kolatsi-JoannouA S Woolf
Sep 21, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Minke van TuylMartin Post
Sep 19, 2008·Journal of the American Society of Nephrology : JASN·Adrian S WoolfDavid A Long
Nov 12, 2005·Nephron. Experimental Nephrology·Joichi UsuiMichio Nagata
Dec 23, 2009·Laboratory Investigation; a Journal of Technical Methods and Pathology·Suzanne McGovernHerman Yeger
Mar 2, 2006·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Guido F LaubeWilliam G van't Hoff
Mar 30, 2016·Current Opinion in Nephrology and Hypertension·Bjoern BuchholzKai-Uwe Eckardt
Mar 28, 2003·Journal of the American Society of Nephrology : JASN·Paul B FreeburgDale R Abrahamson
May 23, 2000·Journal of the American Society of Nephrology : JASN·Hai Tao YuanAdrian S Woolf
Dec 1, 2001·Journal of the American Society of Nephrology : JASN·David A LongHai T Yuan
Aug 14, 1999·Journal of the American Society of Nephrology : JASN·H T YuanA S Woolf
May 18, 2021·Developmental Biology·Anne R RyanOndine Cleaver
Dec 8, 2004·The American Journal of Pathology·Jolanta E PiteraHai Tao Yuan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.