Effects of PCB126 and PCB153 on telomerase activity and telomere length in undifferentiated and differentiated HL-60 cells

Environmental Science and Pollution Research International
Xing XinGabriele Ludewig

Abstract

PCBs are persistent organic pollutants that are carcinogenic and immunotoxic and have developmental toxicity. This suggests that they may interfere with normal cell maturation. Cancer and stem/progenitor cells have telomerase activity to maintain and protect the chromosome ends, but lose this activity during differentiation. We hypothesized that PCBs interfere with telomerase activity and the telomere complex, thereby disturbing cell differentiation and stem/progenitor cell function. HL-60 cells are cancer cells that can differentiated into granulocytes and monocytes. We exposed HL-60 cells to PCB126 (dioxin-like) and PCB153 (nondioxin-like) 6 days before and during 3 days of differentiation. The differentiated cells showed G0/G1 phase arrest and very low telomerase activity. hTERT and hTR, two telomerase-related genes, were downregulated. The telomere shelterins TRF1, TRF2, and POT1 were upregulated in granulocytes, and TRF2 was upregulated and POT1 downregulated in monocytes. Both PCBs further reduced telomerase activity in differentiated cells, but had only small effects on the differentiation and telomere-related genes. Treatment of undifferentiated HL-60 cells for 30 days with PCB126 produced a downregulation of telomerase...Continue Reading

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Citations

Dec 21, 2018·Critical Reviews in Toxicology·Peter MøllerMartin Roursgaard
Jun 24, 2018·Environmental Science and Pollution Research International·Larry W RobertsonNiklas Johansson
Jan 17, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Muhammad Azizan SamadJamilah Syafawati Yaacob
Jan 2, 2021·Environmental Toxicology and Pharmacology·Yaru LiuXinhua Liu

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