Effects of pdgf-bb on rat dermal fibroblast behavior in mechanically stressed and unstressed collagen and fibrin gels

Experimental Cell Research
David I ShreiberR T Tranquillo

Abstract

The dose-response effects of platelet-derived growth factor BB (PDGF-BB) on rat dermal fibroblast (RDF) behavior in mechanically stressed and unstressed type I collagen and fibrin were investigated using quantitative assays developed in our laboratory. In chemotaxis experiments, RDFs responded optimally (P < 0.05) to a gradient of 10 ng/ml PDGF-BB in both collagen and fibrin. In separate experiments, the migration of RDFs and the traction exerted by RDFs in the presence of PDGF-BB (0, 0.1, 1, 10, or 100 ng/ml) were assessed simultaneously in the presence or absence of stress. RDF migration increased significantly (P < 0.05) at doses of 10 and 100 ng/ml PDGF-BB in collagen and fibrin in the presence and absence of stress. In contrast, the effects of PDGF-BB on RDF traction depended on the gel type and stress state. PDGF-BB decreased fibroblast traction in stressed collagen, but increased traction in unstressed collagen (P < 0.05). No statistical conclusion could be inferred for stressed fibrin, but increasing PDGF-BB decreased traction in unstressed fibrin (P < 0.05). These results demonstrate the complex response of fibroblasts to environmental cues and suggest that mechanical resistance to compaction may be a crucial element i...Continue Reading

References

Mar 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·E BellC Merrill
Dec 1, 1990·American Journal of Respiratory Cell and Molecular Biology·A R Osornio-VargasA R Brody
Feb 1, 1990·Experimental Cell Research·D GullbergK Rubin
Jun 1, 1989·Experimental Cell Research·F GrinnellC H Ho
Sep 1, 1989·The Journal of Clinical Investigation·R A ClarkJ M McPherson
Dec 1, 1988·The Journal of Investigative Dermatology·X F GuD Evain-Brion
Sep 1, 1972·The Journal of Cell Biology·T Elsdale, J Bard
Oct 1, 1984·Collagen and Related Research·B NusgensE Bell
Feb 1, 1982·The Journal of Cell Biology·H SeppäG R Martin
Jan 27, 1995·The Journal of Biological Chemistry·Y KatagiriK Tanoue
Mar 27, 1995·Journal of Immunological Methods·P V MogheR T Tranquillo
Mar 1, 1995·Experimental Cell Research·N L Halliday, J J Tomasek
Jul 1, 1994·Annals of Biomedical Engineering·R B DickinsonR T Tranquillo
Sep 1, 1994·The Journal of Cell Biology·V M LathamA F Ross
Feb 1, 1994·The Journal of Cell Biology·F Grinnell
Feb 9, 1996·Cell·D A Lauffenburger, A F Horwitz
Jan 1, 1997·The International Journal of Biochemistry & Cell Biology·P StephensM J Raxworthy
Mar 21, 1998·The American Journal of Surgical Pathology·W SchürchG Gabbiani
Dec 22, 1999·Cell Motility and the Cytoskeleton·A WellsD A Lauffenburger
Aug 3, 2000·Journal of Biomechanical Engineering·T S GirtonR T Tranquillo

❮ Previous
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Citations

Nov 14, 2006·Biomacromolecules·Shaneen L Rowe, Jan P Stegemann
Apr 9, 2011·Journal of Neurotrauma·Harini G SundararaghavanDavid I Shreiber
Feb 7, 2009·Tissue Engineering. Part a·Gary A MonteiroDavid I Shreiber
Nov 14, 2002·Molecular Biology of the Cell·Elisa Tamariz, Frederick Grinnell
Jul 9, 2008·Biomedical Engineering Online·Margaret JuliasDavid I Shreiber
Sep 12, 2009·Experimental & Molecular Medicine·Sangmyung Rhee
Jun 14, 2014·Tissue Engineering. Part B, Reviews·Elizabeth E AntoineMarissa Nichole Rylander
Jul 8, 2003·Cell Motility and the Cytoskeleton·W Matthew Petroll, Lisha Ma
Nov 23, 2006·Journal of Biomedical Materials Research. Part a·Michael C McManusGary L Bowlin
Sep 4, 2008·Biotechnology and Bioengineering·Harini G SundararaghavanDavid I Shreiber
May 3, 2008·Journal of Cellular Physiology·W Matthew PetrollMridula Vishwanath
Jan 9, 2008·Journal of Biomedical Materials Research. Part a·Harini G SundararaghavanDavid I Shreiber
Jan 15, 2011·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Margaret JuliasDavid I Shreiber
Apr 6, 2006·Experimental Cell Research·Kirsty D SmithDouglas A Lauffenburger
May 17, 2006·Acta Biomaterialia·Michael C McManusGary L Bowlin
Feb 25, 2014·Biophysical Journal·Andrew D Rouillard, Jeffrey W Holmes
Jul 18, 2002·The Journal of Surgical Research·Paul A J EneverRobert T Tranquillo
May 29, 2003·Biophysical Journal·David I ShreiberRobert T Tranquillo
Nov 1, 2005·Experimental Cell Research·Frederick GrinnellHongmei Jiang
May 20, 2017·Biomechanics and Modeling in Mechanobiology·Shakti N MenonD L Sean McElwain
May 14, 2003·Trends in Cell Biology·Frederick Grinnell
Nov 28, 2002·International Journal of Impotence Research·T J BivalacquaW J G Hellstrom
Jun 29, 2011·Biomedical Microdevices·Shirley N MasandDavid I Shreiber
Feb 11, 2012·Nanotechnology·G PraveenKrishna Prasad Chennazhi
Feb 28, 2015·Journal of Applied Physiology·Kyle S MartinShayn M Peirce
Mar 8, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Lester Y LeungDaniel J Tschumperlin
Jul 8, 2020·Advances in Wound Care·Shakti N Menon, Jennifer A Flegg
Jun 30, 2019·Integrative Biology : Quantitative Biosciences From Nano to Macro·Quang Long PhamRoman S Voronov
Aug 27, 2018·Cellular and Molecular Bioengineering·Quang Long PhamRoman S Voronov
May 12, 2021·Journal of Zhejiang University. Science. B·Guan WangChunfeng Wang
Feb 19, 2004·Journal of Theoretical Biology·Patrick NamyPhilippe Tracqui
Jun 21, 2005·Medical Engineering & Physics·J Bereiter-Hahn

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