PMID: 7536212Dec 1, 1994Paper

Effects of pentoxifylline and progesterone on human sperm capacitation and acrosome reaction

Human Reproduction
V J KayL Robertson

Abstract

This study was designed to test the effects of pentoxifylline and progesterone upon capacitation of fresh human spermatozoa. Capacitation and acrosomal integrity were assessed using the fluorescent probe chlortetracycline on spermatozoa co-stained with a supravital fluorescent dye, Hoechst 33258. Hyperactivated motility was measured using computer-assisted movement analysis. After exposure to pentoxifylline (1 mg/ml; 30 min), the fluorescent 'B' pattern, characteristic of capacitated, acrosome-intact cells, increased significantly (P < 0.01), though no increase in 'AR' pattern, characteristic of acrosome-reacted cells, was detected. There was a significant increase in hyperactive motility (P < 0.001). Exposure to progesterone (1 microgram/ml; 60 min) resulted in a significant increase in 'B' pattern (P < 0.05) and 'AR' pattern (P < 0.005), though no effect on the expression of hyperactivation was detected. No effect upon hyperactivation was detected on exposure of fresh or cryopreserved spermatozoa to a physiological range of progesterone concentrations (0.1-1000 ng/ml). Sequential exposure to pentoxifylline then progesterone resulted in a significant increase in 'B' pattern, acrosome loss and hyperactivation. Sperm viability w...Continue Reading

Citations

Mar 26, 2003·Reproduction in Domestic Animals = Zuchthygiene·S S Suarez, H-C Ho
Mar 13, 2014·PloS One·Diego Rafael UñatesLaura Cecilia Giojalas
Sep 10, 2017·Reproduction : the Official Journal of the Society for the Study of Fertility·Mayel ChirinosFernando Larrea
Jan 1, 1997·Annual Review of Physiology·M Wehling
Dec 7, 2019·Journal of Animal Science and Biotechnology·MariaPortia B NagataKenichi Yamashita
Feb 1, 1996·International Journal of Andrology·C EmiliozziP Fénichel
Jun 1, 1995·Journal of Endocrinological Investigation·A Lenzi
May 2, 2003·Journal of Andrology·Jan Tesarik, Carmen Mendoza
Oct 31, 2019·Human Reproduction Update·Sean G BrownSarah J Martins da Silva
Jul 28, 2020·Molecular and Cellular Endocrinology·Lara TamburrinoElisabetta Baldi
Mar 28, 2021·Journal of Assisted Reproduction and Genetics·Ebru IbisSinan Ozkavukcu
Feb 9, 2000·Frontiers in Neuroendocrinology·B M SchmidtM Wehling
Jun 16, 2001·Animal Reproduction Science·D A HewittG C England
Jun 25, 2009·Molecular and Cellular Endocrinology·Elisabetta BaldiGianni Forti

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