Effects of peripheral cannabinoid receptor ligands on motility and polarization in neutrophil-like HL60 cells and human neutrophils

The Journal of Biological Chemistry
Rina KuriharaHirohei Yamamura

Abstract

The possible role of the peripheral cannabinoid receptor (CB2) in neutrophil migration was investigated by using human promyelocytic HL60 cells differentiated into neutrophil-like cells and human neutrophils isolated from whole blood. Cell surface expression of CB2 on HL60 cells, on neutrophil-like HL60 cells, and on human neutrophils was confirmed by flow cytometry. Upon stimulation with either of the CB2 ligands JWH015 and 2-arachidonoylglycerol (2-AG), neutrophil-like HL60 cells rapidly extended and retracted one or more pseudopods containing F-actin in different directions instead of developing front/rear polarity typically exhibited by migrating leukocytes. Activity of the Rho-GTPase RhoA decreased in response to CB2 stimulation, whereas Rac1, Rac2, and Cdc42 activity increased. Moreover, treatment of cells with RhoA-dependent protein kinase (p160-ROCK) inhibitor Y27632 yielded cytoskeletal organization similar to that of CB2-stimulated cells. In human neutrophils, neither JWH015 nor 2-AG induced motility or morphologic alterations. However, pretreatment of neutrophils with these ligands disrupted N-formyl-L-methionyl-L-leucyl-L-phenylalanine (fMLP)-induced front/rear polarization and migration and also substantially suppr...Continue Reading

References

May 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·T R BreitmanS J Collins
Oct 4, 1995·Biochemical and Biophysical Research Communications·T SugiuraK Waku
Aug 23, 1996·The Journal of Biological Chemistry·M AmanoK Kaibuchi
Feb 2, 1999·The EMBO Journal·F Sánchez-Madrid, M A del Pozo
Dec 7, 2000·Chemistry and Physics of Lipids·E V Berdyshev
Jun 8, 2002·Prostaglandins, Leukotrienes, and Essential Fatty Acids·T SugiuraK Waku
Dec 12, 2002·Molecular Biology of the Cell·Robert J EddyFrederick R Maxfield
May 22, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Michael GlogauerDavid J Kwiatkowski
Sep 10, 2003·Journal of Leukocyte Biology·Thomas W KleinHerman Friedman
Sep 11, 2003·Journal of Neuroimmunology·Engelbert DeuschHans Georg Kress
Nov 6, 2003·Journal of Cell Science·Nancy HoggAlison McDowall
Mar 23, 2004·Cancer Immunology, Immunotherapy : CII·Jan JosephFrank Entschladen
Sep 16, 2004·Nature Reviews. Molecular Cell Biology·Peter J M Van Haastert, Peter N Devreotes
Oct 28, 2004·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·S M Shahjahan MiahKiyonao Sada
Feb 15, 2005·Biochemical and Biophysical Research Communications·Satoshi MatsusakaHirohei Yamamura
Mar 9, 2005·Trends in Cell Biology·Gary M Bokoch
Mar 29, 2005·Nature Cell Biology·Zhong LiDianqing Wu
Apr 30, 2005·Proceedings of the National Academy of Sciences of the United States of America·Jingsong XuHenry R Bourne
Jul 19, 2005·Journal of Neuroimmunology·J Ludovic Croxford, Takashi Yamamura
Oct 26, 2005·Current Biology : CB·Xuemei DongDianqing Wu

❮ Previous
Next ❯

Citations

Jun 1, 2013·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Rodrigo GuabirabaMauro M Teixeira
Mar 9, 2013·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Slava Rom, Yuri Persidsky
Apr 7, 2011·Cell Research·Nariman A B BalengaMaria Waldhoer
Oct 3, 2007·Immunology and Cell Biology·Akiko ShiratsuchiYoshinobu Nakanishi
Mar 24, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Servio H RamirezYuri Persidsky
Apr 13, 2011·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Nicole J LeCapitainePatricia E Molina
Jun 16, 2014·Neuromolecular Medicine·Esmée Vendel, Elizabeth C M de Lange
Apr 10, 2009·Seminars in Immunopathology·Pál Pacher, Sabine Steffens
Dec 27, 2011·Progress in Neuro-psychopharmacology & Biological Psychiatry·Brady K AtwoodKen Mackie
Jan 24, 2009·Journal of Molecular and Cellular Cardiology·Fabrizio MontecuccoSabine Steffens
Apr 9, 2008·Molecular and Cellular Endocrinology·Tibor HarkanyJan Mulder
Jun 11, 2015·Journal of Medical Virology·Alireza TahamtanVahid Salimi
Oct 2, 2007·British Journal of Pharmacology·C A LunnL Bober
Nov 6, 2007·British Journal of Pharmacology·A M Miller, N Stella
Oct 1, 2013·Progress in Lipid Research·Ismael Galve-RoperhMauro Maccarrone
Sep 20, 2006·Expert Opinion on Therapeutic Targets·Charles A LunnLoretta Bober
Jul 31, 2013·Drug Discovery Today·Mireille AlhouayekGiulio G Muccioli
Feb 4, 2014·Biochemical and Biophysical Research Communications·Wang SuijunWang Yanfang
Mar 10, 2012·PloS One·Mohamed AbdgawadThomas Hellmark
Jul 6, 2016·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Magaiver Andrade-SilvaMaria G Henriques
Oct 23, 2016·Cellular and Molecular Life Sciences : CMLS·Caroline TurcotteNicolas Flamand
Oct 4, 2016·Frontiers in Pharmacology·Caroline TurcotteNicolas Flamand
Nov 12, 2016·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Susan FungNephi Stella
Jan 15, 2015·Immunology·Valerio ChiurchiùMauro Maccarrone
Feb 1, 2016·Cannabis and Cannabinoid Research·Valerio Chiurchiù
Feb 26, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Theodore S KapellosAsif J Iqbal
Jan 28, 2009·Journal of Biomolecular Screening·Debra McGuinnessCharles A Lunn
Jul 1, 2014·Journal of Cardiovascular Pharmacology·Yan LuHope D Anderson
Jun 24, 2020·Journal of Leukocyte Biology·Mariantonia BraileStefania Loffredo
Feb 1, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·François ChouinardNicolas Flamand
Aug 14, 2018·The Journal of Clinical Investigation·Rose L SzabadyBeth A McCormick
Dec 22, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Turgut Emrah Bozkurt
Jul 27, 2006·The Journal of Biological Chemistry·Matthieu De RivoyreIsabelle Mus-Veteau
Nov 4, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sreemanti BasuBonnie N Dittel

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.