Effects of peroxisome proliferator-activated receptor gamma ligands ciglitazone and 15-deoxy-delta 12,14-prostaglandin J2 on rat cultured cerebellar granule neuronal viability

Journal of Neuroscience Research
S A SmithS J Roberts-Thomson

Abstract

Peroxisome proliferator-activated receptor gamma (PPARgamma) has been the focus of studies assessing its potential neuroprotective role. These studies have shown either neuroprotection or neurotoxicity by PPARgamma ligands. Comparison of these studies is complicated by the use of different PPARgamma ligands, mechanisms of neurotoxicity induction, and neuronal cell type. In this study, we compared the effects of the synthetic PPARgamma ligand ciglitazone with an endogenous PPARgamma ligand, 15-deoxy-delta(12,14)-prostaglandin J(2) (15-deoxy PGJ(2)), on inherent neurotoxicity and neuroprotection using a reduction in extracellular KCl in rat cultured cerebellar granule neurons (CGN). We also assessed the effects of these ligands on c-Jun protein expression, which is up-regulated on induction of low-KCl-mediated neuronal apoptosis as well as being associated with PPAR in other cell types. We showed that PPARgamma mRNA is expressed in CGN cultures and observed ciglitazone- and 15-deoxy PGJ(2)-mediated inherent neurotoxicity that was concentration and time dependent. c-Jun was only modestly increased in the presence of ciglitazone but was markedly up-regulated by 15-deoxy PGJ(2) after 12 hr. Treatment of CGN cultures with ciglitazone...Continue Reading

References

Dec 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·S R D'MelloP Calissano
Apr 29, 1998·Journal of Neuroscience Research·N S CheungS F Giardina
Oct 31, 1998·Current Opinion in Genetics & Development·S A Kliewer, T M Willson
Apr 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·D S StrausC K Glass
Sep 29, 2000·Biochemical Pharmacology·J HamB Shah
Apr 17, 2001·Journal of Molecular Neuroscience : MN·F J Martín-RomeroP Fernandez-Salguero
Jul 19, 2001·The Journal of Biological Chemistry·E D Rosen, B M Spiegelman
Aug 29, 2001·Journal of Investigative Medicine : the Official Publication of the American Federation for Clinical Research·C E ClayF H Chilton
Aug 31, 2001·Biochemical and Biophysical Research Communications·F YinR E Law
Dec 18, 2001·Journal of Neuroscience Research·P PiccioliG Schettini
Apr 17, 2002·Cellular Signalling·Olivier Nosjean, Jean A Boutin
May 16, 2002·Journal of Cellular Physiology·Satoru KawakamiKazunori Kihara
May 29, 2002·Proceedings of the National Academy of Sciences of the United States of America·Mitsuhiro KondoKoji Uchida

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Citations

Mar 12, 2004·Brain Research. Developmental Brain Research·Eric ParadisM R Ven Murthy
Sep 24, 2014·PPAR Research·Rodrigo A QuintanillaFabián A Cabezas-Opazo
Mar 26, 2016·Frontiers in Neuroscience·Margarita Pérez-MartínJuan Suárez
Aug 10, 2007·Expert Opinion on Pharmacotherapy·Roger S McIntyreSidney H Kennedy
Jul 8, 2008·PPAR Research·Paul D DrewMichael K Racke
Apr 10, 2007·Trends in Pharmacological Sciences·Juraj CulmanThomas Herdegen
Jun 24, 2004·Journal of Neuroscience Research·Steven A SmithSarah J Roberts-Thomson
May 25, 2005·The Journal of Biological Chemistry·Won Jae LeeGregory R Monteith
Jun 14, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yi ZhaoJuraj Culman
Nov 29, 2014·CNS Neuroscience & Therapeutics·Xiu-Rong ZhaoJaroslaw Aronowski

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