Effects of peroxisome proliferator-activated receptor alpha activation on pathways contributing to cholesterol homeostasis in rat hepatocytes

Biochimica Et Biophysica Acta
Catherine Le Jossic-CorcosPaulette Bournot

Abstract

Peroxisome proliferator-activated receptor alpha (PPARalpha) activation by fibrates controls expression of several genes involved in hepatic cholesterol metabolism. Other genes could be indirectly controlled in response to changes in cellular cholesterol availability. To further understand how fibrates may affect cholesterol synthesis, we investigated in parallel the changes in the metabolic pathways contributing to cholesterol homeostasis in liver. Ciprofibrate increased HMG-CoA reductase and FPP synthase mRNA levels in rat hepatocytes, together with cholesterogenesis from [(14)C] acetate and [(3)H] mevalonate. The up-regulation observed in fenofibrate- and WY-14,643-treated mice was abolished in PPARalpha-null mice, showing an essential role of PPARalpha. Among the three sterol regulatory element-binding protein (SREBP) mRNA species, only SREBP-1c level was significantly increased. In ciprofibrate-treated hepatocytes, cholesterol efflux was decreased, in parallel with cholesteryl ester storage and bile acids synthesis. As expected, AOX expression was strongly induced, supporting evidence of the peroxisome proliferation. Taken together, these results show that fibrates can cause cholesterol depletion in hepatocytes, possibly i...Continue Reading

References

Mar 1, 1992·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·B StaelsJ Auwerx
Feb 1, 1991·Archives of Biochemistry and Biophysics·H Hayashi, S Takahata
Jan 1, 1991·Journal of Clinical Pharmacology·P ZimetbaumS Kahn
Nov 1, 1989·Archives of Biochemistry and Biophysics·H Hayashi, A Miwa
Jul 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·M S BrownJ L Goldstein
Nov 10, 1998·Circulation·B StaelsJ C Fruchart
Oct 26, 1999·Endocrine Reviews·B Desvergne, W Wahli
Feb 26, 2000·Journal of Medicinal Chemistry·T M WillsonB R Henke
Mar 29, 2000·Biochemical Pharmacology·F HashimotoH Hayashi
Nov 22, 2000·The Journal of Biological Chemistry·V C HannahM S Brown
Mar 27, 2001·Current Opinion in Lipidology·G Schmitz, T Langmann
Sep 12, 2001·Biochemical Pharmacology·N RoglansJ C Laguna
Dec 4, 2001·Biochimica Et Biophysica Acta·Q GuoY Chao
May 15, 2002·Arteriosclerosis, Thrombosis, and Vascular Biology·O BarbierB Staels
Jun 5, 2003·Biochimica Et Biophysica Acta·Jeffrey M PetersFrank J Gonzalez
Apr 16, 2004·The Journal of Steroid Biochemistry and Molecular Biology·Catherine Le Jossic-CorcosPaulette Bournot

❮ Previous
Next ❯

Citations

May 5, 2011·The Journal of Biological Chemistry·Ana Fernández-AlvarezMarta Casado
Jun 21, 2005·Journal of Cardiovascular Pharmacology·Vanessa DeclercqMohammed H Moghadasian
Aug 11, 2012·Bioscience, Biotechnology, and Biochemistry·Yoshiki InoueKazuo Kobayashi-Hattori
May 13, 2014·Free Radical Biology & Medicine·Pin-Ho PanChun-Jung Chen
Aug 12, 2014·The Journal of Nutritional Biochemistry·Shih-Yi LinChun-Jung Chen
Sep 8, 2005·Atherosclerosis·Dwayne E DoveSergio Fazio
Feb 29, 2008·Reproduction : the Official Journal of the Society for the Study of Fertility·David GunnarssonGunnar Selstam
Oct 12, 2004·The Biochemical Journal·Catherine Le Jossic-CorcosPaulette Bournot
Aug 2, 2005·Cell Metabolism·Manu V ChakravarthyClay F Semenkovich
Oct 12, 2005·The Journal of Nutritional Biochemistry·Bettina König, Klaus Eder
Sep 9, 2008·Biochimica Et Biophysica Acta·Katrin MartensMyriam Baes
Apr 19, 2011·The Journal of Nutritional Biochemistry·Wen-Ying ChenChun-Jung Chen
Jan 18, 2012·The International Journal of Biochemistry & Cell Biology·Fabrizio DamianoLuisa Siculella

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.