Effects of pH and calcium ions on the conformational transitions in silk fibroin using 2D Raman correlation spectroscopy and 13C solid-state NMR

Biochemistry
Ping ZhouWen-Hua Yao

Abstract

Silk fibroin exists in a number of different states, such as silk I and silk II, with different properties largely defined by differences in secondary structure composition. Numerous attempts have been made to control the transitions from silk I to silk II in vitro to produce high-performance materials. Of all the factors influencing the structural compositions, pH and some metal ions play important roles. This paper focuses on the influence of pH and Ca(2+) ions on the conformational transition from silk I to silk II in regenerated (redissolved) Bombyx mori fibroin. One- and two-dimensional correlation Raman spectroscopy was used to describe qualitatively the transitions in secondary structure in silk I, silk II, and their intermediates as pH and Ca(2+) ion concentration were changed, while (13)C cross polarization magic angle spinning (CP/MAS) solid-state NMR was used to quantify these changes. We showed that conditions (low pH, pH 5.2; a defined range of Ca(2+) ion concentrations; gradual water removal) that mimic natural silk spinning promote the formations of beta-sheet and distorted beta-sheet characteristic of silk II or silk II-related intermediate. In contrast, higher pH (pH 6.9-8.0) and higher Ca(2+) ion concentration...Continue Reading

References

Mar 16, 1989·Biochimica Et Biophysica Acta·A Apap-BolognaG Kemp
Jan 5, 1996·Science·D A Tirrell
Jul 29, 1998·Journal of Molecular Biology·R TumaP E Prevelige
Mar 29, 2000·FEBS Letters·H LilieU Baumann
Jul 26, 2002·Journal of the American Chemical Society·Tetsuo AsakuraAnne S Ulrich
Aug 16, 2002·Nature·Zhengzhong Shao, Fritz Vollrath
Dec 14, 2002·The Journal of Biological Chemistry·Kimberly A McClintock, Gary S Shaw
Aug 9, 2003·Journal of the American Chemical Society·Amalia AggeliNeville Boden

❮ Previous
Next ❯

Citations

Nov 26, 2015·Acta Biomaterialia·Sonia Kapoor, Subhas C Kundu
Mar 19, 2013·Carbohydrate Polymers·Songmin ShangJintu Fan
Aug 7, 2010·International Journal of Biological Macromolecules·Juan ZhouJing Lin
Dec 15, 2015·Materials Science & Engineering. C, Materials for Biological Applications·Jinfa MingFukui Pan
Jul 17, 2007·Journal of Bioscience and Bioengineering·Rikako KinoMasanobu Munekata
Jul 12, 2016·ACS Biomaterials Science & Engineering·Davoud EbrahimiMarkus J Buehler
Jul 25, 2006·International Journal of Biological Macromolecules·Xin-Xing FengChang-Ian Jia
Apr 12, 2016·Microscopy Research and Technique·Wenhua LiJuming Yao
Sep 1, 2012·International Journal of Biological Macromolecules·Yichun HangXuechao Hu
Feb 14, 2013·International Journal of Biological Macromolecules·Suna FanXuechao Hu
Jun 29, 2010·Methods : a Companion to Methods in Enzymology·Victor A ShashilovIgor K Lednev
Jan 5, 2010·Journal of Structural Biology·Markus HeimThomas Scheibel
Apr 23, 2016·Scientific Reports·Zhaoming DongQingyou Xia
Oct 27, 2016·Journal of the Science of Food and Agriculture·Xiaoxiao TangKang Sun
Jul 10, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Nicholas N Ashton, Russell J Stewart
Nov 7, 2019·Biological Trace Element Research·Anurupa Goswami, Dipali Devi
Sep 1, 2020·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Sharda GuptaArindam Bit
Jan 17, 2013·Chemical Communications : Chem Comm·Hui WangHongyao Xu
Dec 14, 2016·Annual Review of Entomology·Sean J BlamiresI-Min Tso
Aug 19, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ima GhaeliFernando J Monteiro
Jan 16, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hiromitsu SogawaKeiji Numata
Jun 27, 2019·International Journal of Molecular Sciences·Qingsong LiuQingyou Xia
Oct 28, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·N DrnovšekS Novak
Feb 18, 2021·Biomedical Materials·Maryam FarokhiHeinz Redl
May 1, 2021·International Journal of Molecular Sciences·Meihui ZhaoShenzhou Lu
Jul 12, 2021·Advanced Materials·Firoz Babu KadumudiAlireza Dolatshahi-Pirouz

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cajal Bodies & Gems

Cajal bodies or coiled bodies are dense foci of coilin protein. Gemini of Cajal bodies, or gems, are microscopically similar to Cajal bodies. It is believed that Cajal bodies play important roles in RNA processing while gems assist the Cajal bodies. Find the latest research on Cajal bodies and gems here.