Effects of pH manipulation, CatSper stimulation and Ca2+-store mobilization on [Ca2+]i and behaviour of human sperm

Human Reproduction
Cosmas AchikanuStephen Publicover

Abstract

How do the alkaline pH, progesterone and 4-aminopyridine interact in their effects on human sperm? Behaviour of human sperm (proportion of hyperactivated cells and motility kinematics) were related directly to [Ca2+]i irrespective of pH or the agonist applied. CatSper channels of human sperm, which are central to generation of sperm [Ca2+]i signals and induction of hyperactivated motility, are activated by intracellular alkalinization and progesterone. Progesterone (P4) is much less effective than 4-aminopyridine (4-AP) (which mobilizes stored Ca2+ but also raises pHi) as an inducer of hyperactivation. This was a laboratory study, spanning ~18 months that used 15 sperm donors and involved more than 100 separate experiments. Semen donors and patients were recruited in accordance with local ethics approval (ERN_12-0570R). [Ca2+]i responses of suspended cell populations were examined by fluorimetric recording and motility parameters assessed by computer-assisted sperm analysis. Increasing pHo from 7.4 to 8.5 raised pHi (from 6.9 to 7.2) and significantly increased both [Ca2+]i and the proportion of hyperactivated cells. Stimulation of cells with P4 (1 nM-20 μM) induced a biphasic (transient and plateau) increase in [Ca2+]i. The [C...Continue Reading

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Citations

Jun 14, 2019·Molecular Human Reproduction·Cosmas AchikanuStephen Publicover
Oct 31, 2019·Human Reproduction Update·Sean G BrownSarah J Martins da Silva
Dec 22, 2019·Scientific Reports·Sergio A MachadoDavid J Miller
Feb 6, 2020·Frontiers in Cell and Developmental Biology·Julio C ChávezTakuya Nishigaki
Oct 19, 2021·Frontiers in Cell and Developmental Biology·Qifu HeFusheng Quan

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