Abstract
Ex-situ biogas upgrading based on hydrogenotrophic methanogenic process has attracted much attention with the depletion of fossil fuels. Consumption of CO2 leads to the pH increase in the mixed cultures of biogas upgrading system. The hydrogenotrophic methanogens were enriched at pH 5.5-6.0, 7.0-7.5, and 8.5-9.0 and at 55°C and 70°C. The methane production activity and microbial community structure were evaluated. Semi-continuous experimental results showed that stable and similar methane production was obtained at pH 7.0-7.5 and 8.5-9.0. In addition, pH 8.5-9.0 presented higher maximum methane production rate compared to pH 7.0-7.5. pH below 6 obtained the longest lag phase time of about 17.4 h, more than twice the values at pH 7.0-7.5 (8.8 h) and pH 8.5-9.0 (6.9 h) at 55°C. The predominant methanogen was the genus Methanothermobacter, a hydrogenotrophic methanogen at higher temperatures. Methanobacterium became predominant at pH 8.5-9.0 and the abundance increased to 83.6% at 55°C. Coprothermobacter and Caldanaerobacter were identified as the core functional bacteria under alkaline condition and were likely involved in syntrophic acetate oxidation with hydrogenotrophic methanogens.
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