Effects of pH on responses to adenosine, CGS 21680, carbachol and nitroprusside in the isolated perfused superior mesenteric arterial bed of the rat

British Journal of Pharmacology
C R HileyP J Richardson

Abstract

1. The receptors mediating the vasodilator responses to adenosine in the isolated mesenteric arterial bed of the rat were identified by use of selective agonists and antagonists and the involvement of the endothelium was examined. 2. Adenosine-mediated dilatation of the mesentery was potentiated by the nitric oxide synthase inhibitor, NG-nitro-L-arginine methyl ester (L-NAME, 100 microM), but in contrast, removal of the endothelium substantially reduced the responses to adenosine. 3. The order of potency of adenosine receptor agonists was: 5'-N-ethylcarboxamidoadenosine (NECA) > 2-p-(-2-carboxyethyl)phenethylamino-5'-N-ethylcarboxamidoadenosine (CGS 21680) > 2-chloro-N6-cyclopentyl-adenosine (CCPA) > or = adenosine, suggesting the presence of A2A receptors. 4. Adenosine-mediated dilatation was inhibited by the non-selective adenosine receptor antagonist, 8-phenyltheophylline (3 microM) and by the A2A receptor antagonist 8-(3-chlorostyryl)caffeine (500 nM), but was unaffected by the A1 receptor antagonist, 1,3-dipropyl-8-cyclopentylxanthine (DPCPX; 10 nM). 5. Reducing the pH of the perfusate to 6.8 potentiated the actions of both CGS 21680 and adenosine, but the vasodilator effects of carbachol were the same at both pH values. T...Continue Reading

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Citations

Apr 30, 2008·Purinergic Signalling·Manuela MoratoAntónio Albino-Teixeira
Jan 30, 2004·Vascular Pharmacology·Worku Abebe, Mahmood S Mozaffari
Dec 12, 2001·Toxicon : Official Journal of the International Society on Toxinology·Steven D Aird
Dec 1, 2001·Pharmacology & Therapeutics·R Tabrizchi, S Bedi
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Jan 5, 2013·Journal of Cardiovascular Pharmacology·Bunyen TengS Jamal Mustafa
Jun 24, 2008·Microscopy Research and Technique·Sandra LealCarmen Diniz
Aug 8, 2007·International Journal of Urology : Official Journal of the Japanese Urological Association·Kazuyoshi KataokaHiromichi Tsuru
Feb 12, 2013·European Journal of Pharmacology·Hanan M El-GowelliMahmoud M El-Mas
Dec 20, 2017·Frontiers in Pharmacology·Allan K N AlencarGisele Zapata-Sudo
Dec 18, 2013·Pharmacological Reviews·Geoffrey Burnstock, Vera Ralevic
Mar 13, 2014·The International Journal of Artificial Organs·Ahmed NassarCristiano Quintini
Mar 13, 2015·Molecular Pharmacology·Guillaume LebonChristopher G Tate
Jun 13, 2002·American Journal of Physiology. Renal Physiology·Edwin K JacksonStevan P Tofovic
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