PMID: 9550292Apr 29, 1998Paper

Effects of pharmacological inhibition of glutamate-uptake on ischaemia-induced glutamate efflux and anoxic depolarization latency

Naunyn-Schmiedeberg's Archives of Pharmacology
T P ObrenovitchJ Urenjak

Abstract

It has been proposed that deficient glutamate uptake, by increasing the extracellular concentration of this excitatory neurotransmitter, may contribute to the pathophysiology of cerebral ischaemia. This study aimed to examine whether pharmacological inhibition of glutamate uptake altered the kinetics of ischaemia-induced glutamate efflux, and precipitated anoxic depolarisation. Microdialysis was used for application of the glutamate-uptake inhibitor L-trans-pyrrolidine-2,4-dicarboxylate (L-trans-PDC), recording of the EEG and extracellular direct current (DC) potential with an electrode within the probe, and continuous monitoring of changes in extracellular glutamate. L-trans-PDC was applied locally from 8 min prior to cardiac arrest to the end of the recording period. L-trans-PDC (2.5 mM) barely altered the time course of postmortem glutamate efflux in the cortex. Only the maximum rate of efflux during the first exocytotic phase, and the concentration reached at the end of this phase, appeared slightly increased. L-trans-PDC (5 mM) reduced significantly the delay between EEG silence and anoxic depolarization in the cerebral cortex (59.2 +/- 9.2 s vs. 79.7 +/- 11.5 s; n = 6), but not in the striatum and hippocampus. These effec...Continue Reading

Citations

May 14, 2003·Neurochemistry International·J W Phillis, M H O'Regan
Mar 15, 2000·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·T P ObrenovitchT M Jay
Mar 19, 1999·Clinical and Experimental Pharmacology & Physiology·I WesslerK Racké
May 20, 1999·Annual Review of Pharmacology and Toxicology·R P Seal, S G Amara
Apr 26, 2002·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Christine GebhardtUwe Heinemann
Nov 6, 2014·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Jake T NeumannMiguel A Perez-Pinzon
Feb 13, 2001·Methods : a Companion to Methods in Enzymology·T P Obrenovitch, E Zilkha
Jan 5, 2000·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·N G HarrisS R Williams
Feb 5, 1999·Stroke; a Journal of Cerebral Circulation·Y SekiH K Kimelberg
Apr 18, 2002·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Sarah L MiltonPeter L Lutz

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