PMID: 8939475Oct 25, 1996Paper

Effects of phorbol ester on intracellular Ca2+ and membrane currents in cultured human microglia

Neuroscience Letters
A S YooS U Kim

Abstract

The effects of protein kinase C (PKC) activation by phorbol ester on intracellular Ca2+ concentration ([Ca2+]i) and membrane currents in human microglia grown in culture were investigated. Treatment of microglia with phorbol myristate acetate (PMA) resulted in a large increase in [Ca2+]i in cells loaded with fura-2. The increased levels of [Ca2+]i were not altered following removal of the phorbol ester. In Ca(2+)-free medium, application of PMA did not increase [Ca2+]i. In addition, PMA application in standard Ca(2+)-solution containing lanthanum (1.8 mM) had no effect on the microglial response to PMA, suggesting that the phorbol ester actions were due to transmembrane influx of Ca2+ but not through voltage-gated Ca2+ channels. Whole-cell patch clamp measurements demonstrated that PMA potentiated an outward K+ current and inhibited an inward rectifier K+ current. This study is the first demonstration that PKC activation by phorbol ester leads to increased intracellular [Ca2+] and changes in membrane currents in human microglia.

Citations

Dec 5, 1998·Neuroscience Letters·L ZhangC Krieger
Jun 17, 1998·The Journal of General Physiology·W ZhouT E DeCoursey
Oct 16, 2002·Glia·Thomas Möller
Sep 22, 2015·IEEE Transactions on Pattern Analysis and Machine Intelligence·Marcello Demi
Aug 4, 1998·The American Journal of Physiology·C Eder
Jul 13, 1999·Microscopy Research and Technique·P Rezaie, D Male
Jan 5, 2000·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·S KoponenJ Koistinaho
Mar 14, 2001·American Journal of Physiology. Cell Physiology·R KhannaL C Schlichter

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