PMID: 11897848Mar 19, 2002Paper

Effects of phosphocreatine on SR Ca(2+) regulation in isolated saponin-permeabilized rat cardiac myocytes

The Journal of Physiology
Zhaokang Yang, D S Steele

Abstract

The effects of phosphocreatine (PCr) on sarcoplasmic reticulum (SR) Ca(2+) regulation were investigated in saponin-permeabilized rat ventricular myocytes. Cells were perfused continuously with weakly Ca(2+)-buffered solutions approximating to the intracellular milieu. Ca(2+) release from the SR was detected using Fura-2 or Fluo-3. Withdrawal of PCr reduced the frequency of spontaneous Ca(2+) release by 12.8 +/- 3.4 % (n = 9) and the amplitude of the spontaneous Ca(2+) transient by 17.4 +/- 3.1 % (n = 9). Stepwise reductions in [PCr] progressively increased the time for the spontaneous Ca(2+) transient to rise from 25 to 100 % of the maximum value (TP75) and to fall by 75 % of the peak level (DT75). Following complete PCr withdrawal, the TP75 and the DT75 were 147.1 +/- 13.2 and 174.8 +/- 23.2 % of the control values, respectively. Experiments involving confocal microscopy showed that PCr withdrawal decreased the propagation velocity of spontaneous Ca(2+) waves. PCr withdrawal also reduced the frequency and amplitude, but increased the duration of spontaneous Ca(2+) sparks. Rapid application of 20 mM caffeine was used to assess the SR Ca(2+) content at the point of spontaneous Ca(2+) release. In the absence of PCr, the amplitude...Continue Reading

References

Aug 1, 1975·The Journal of Physiology·A Fabiato, F Fabiato
Aug 1, 1992·European Journal of Biochemistry·M Arrio-DupontA d'Albis
Feb 1, 1987·Circulation Research·D G Allen, C H Orchard
Jan 1, 1984·The American Journal of Physiology·D J Miller, G L Smith
May 1, 1995·The American Journal of Physiology·J W BassaniD M Bers
Feb 1, 1994·The Journal of Membrane Biology·R Sitsapesan, A J Williams
May 1, 1993·European Journal of Biochemistry·P KorgeK B Campbell
Sep 1, 1996·Pflügers Archiv : European journal of physiology·A MinajevaV Veksler
Jan 1, 1996·The American Journal of Physiology·H ChengM B Cannell
Oct 1, 1996·Pflügers Archiv : European journal of physiology·V LukyanenkoS Györke
Apr 15, 1997·The Journal of Physiology·S GyörkeI Györke
Jul 31, 1998·The American Journal of Physiology·M KawaiC H Orchard
Jul 13, 1999·Circulation Research·E De SousaR Ventura-Clapier
Dec 22, 1999·The Journal of Physiology·V Lukyanenko, S Gyorke
Dec 22, 1999·Annals of the New York Academy of Sciences·S E LehnartG Hasenfuss
Jan 30, 1971·FEBS Letters·M Makinose, W Hasselbach

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Citations

Apr 2, 2004·Neurobiology of Disease·Peter KlivenyiM Flint Beal
Aug 28, 2003·Acta Physiologica Scandinavica·D S Steele, A M Duke
Feb 8, 2013·PloS One·Enric Alvarez-LacalleBlas Echebarria
Apr 20, 2017·Oxidative Medicine and Cellular Longevity·Valery I KapelkoVladimir P Shirinsky

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