Effects of phosphorylation by protein kinase CK2 on the human basal components of the RNA polymerase II transcription machinery

Journal of Cellular Biochemistry
María Eugenia CabrejosE Maldonado

Abstract

We have investigated the role of phosphorylation by vertebrate protein kinase CK2 on the activity of the General Transcription Factors TFIIA, TFIIE, TFIIF, and RNAPII. The largest subunits of TFIIA, TFIIE, and TFIIF were phosphorylated by CK2 holoenzyme. Also, RNA polymerase II was phosphorylated by CK2 in the 214,000 and 20,500 daltons subunits. Our results show that phosphorylation of TFIIA, TFIIF, and RNAPII increase the formation of complexes on the TATA box of the Ad-MLP promoter. Also, phosphorylation of TFIIF increases the formation of transcripts, where as phosphorylation of RNA polymerase II dramatically inhibits transcript formation. Furthermore, we demonstrate that CK2 beta directly interacts with RNA polymerase II, TFIIA, TFIIF, and TBP. These results strongly suggest that CK2 may play a role in regulating transcription of protein coding genes.

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Citations

Nov 21, 2007·Oncogene·S LehnertM Montenarh
Apr 20, 2014·PloS One·Hyeongki KimSungchan Cho
Dec 3, 2014·Journal of Proteomics·Nicole St-DenisDavid W Litchfield
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Jun 22, 2012·Trends in Neurosciences·Steven J Coultrap, K Ulrich Bayer
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Sep 25, 2017·The Journal of Biological Chemistry·Teresita Padilla-BenavidesAnthony N Imbalzano
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Jun 13, 2008·Molecular and Cellular Biochemistry·Nerea Allende-VegaDavid Meek
Mar 7, 2014·The Journal of Biological Chemistry·Melissa A Mullen DavisDonal S Luse
Jul 11, 2013·Chemical Reviews·Célia JeronimoFrançois Robert

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